PhD Position: Optimisation of Protocol and Commercial Kit for Quantification of Mitochondrial DNA Oxidative Lesions

This funded PhD position at the University of Udine, Department of Medicine, is part of the INT2ACT Doctoral Network and focuses on developing and optimising a protocol for quantifying oxidative lesions in mitochondrial DNA (mtDNA). The project aims to translate this protocol into a standardized commercial kit, addressing a critical need for sensitive and reproducible methods to detect mtDNA oxidative damage—a key factor in neurodegenerative diseases and other pathological conditions. The research will integrate advanced molecular biology techniques, including qPCR-based assays, DNA damage quantification, and lesion-specific polymerase arrest assays, to measure oxidative modifications in mtDNA. Initial work will refine and validate the protocol in standard biological models, followed by adaptation for complex systems such as neuronal cell models relevant to neurodegeneration. A major goal is to produce technical guidelines for applying the protocol across species and experimental contexts. The final phase will focus on high-throughput and commercial adaptation, ensuring scalability, reproducibility, and user-friendliness. The host laboratory, led by Dr. Carlo Vascotto, specializes in DNA repair mechanisms, mitochondrial RNA processes, and the role of mitochondria in tumor progression and resistance, with access to state-of-the-art facilities for cell culture, flow cytometry, confocal and nanoscope microscopy, and in vivo fluorescence imaging. The project includes secondments to collaborating groups in Germany (Prof. Lena Burbulla, Ludwig Maximilian University; Dr. Thomas Frischmuth, baseclick GmbH), requiring willingness to travel and spend time abroad. Eligibility requires a Master’s degree in Biology, Biotechnology, or related field, compliance with the mobility rule, and proficiency in English. Applicants should have research experience in cellular and molecular biology, with mitochondrial biology and nucleic acids analysis as strong assets. Application involves submitting a statement of interest, CV, examination certificate, and degree certificate (in English) as a single PDF. The deadline is January 31, 2026. For more information, visit the INT2ACT project website or contact Dr. Carlo Vascotto.