Organelles are specialized subcellular compartments, such as mitochondria (the cell's powerhouse) and chloroplasts (which enable photosynthesis). Creating custom organelles for specific purposes remains a challenge. Therefore, the Rinaldi lab is using engineering biology to create synthetic organelles with various biotechnological applications. Following advances in DNA sequencing and manipulation, synthetic organelles could represent the next major breakthrough in synthetic biology.

This PhD project focuses on engineering synthetic organelles to compartmentalize oily chemicals in microbes, using components from plants and other organisms. The goal is to tackle chemical toxicity in biomanufacturing—a process that uses microbial fermentation to produce chemicals. Synthetic organelles could significantly enhance chemical tolerance and boost the production of high-value chemicals.

The project's primary application is producing sustainable aviation fuels from waste cellulose, aligning with sustainability and renewable carbon goals. The multidisciplinary supervisory team, combining expertise in Molecular Biology, Plant Science, and Chemical Engineering, collaborates with two industrial partners to build a supply chain from feedstock to biofuel producer. This project aims to achieve industrial symbiosis and a circular bioeconomy. Additionally, Techno-Economic Assessment and Life Cycle Analysis will assess the technology’s commercial viability and sustainability, targeting Technology Readiness Level 4 for commercialisation.

PhD candidates will join a vibrant and supportive research community receiving mentorship, technical support, and expertise, and through regular lab meetings and seminars. They will gain training in a unique skillset that bridges molecular biology and fermentation science, addressing skill gaps in the UK's growing bioeconomy. They will produce impactful publications and patents, explore diverse post-PhD career paths, and engage with a wide range of disciplines and stakeholders, preparing them for opportunities in academia, industry, biotech entrepreneurship, civic society, and beyond.