PhD Studentship: Next-Generation Lateral Flow Assays for Rapid Detection of Respiratory Tract Infections and Antimicrobial Resistance

[Stipend at UKRI rate; funding includes stipend and likely covers tuition as per UKRI studentship norms.] Project Overview: This PhD studentship at University College London (UCL) focuses on developing next-generation lateral flow assays (LFAs) for the rapid and ultrasensitive detection of respiratory tract infection (RTI) pathogens and antimicrobial resistance (AMR) markers. RTIs are a major driver of antibiotic prescriptions and AMR globally, and the lack of rapid diagnostics leads to empirical antibiotic use, exacerbating resistance. The project aims to address this challenge by creating a novel LFA platform that integrates plasmonic signal amplification using gold nanostars with a power-free electrokinetic focusing mechanism, enabling early-stage detection without external instrumentation. Research Objectives and Methods: The student will synthesize gold nanostars with optimized optical and surface properties to enhance plasmonic signal amplification. The project involves engineering LFA architectures that incorporate salt-gradient-driven diffusiophoretic focusing for power-free analyte concentration. Nanomaterials and microfluidic components will be integrated into scalable, user-friendly LFA prototypes. Device performance will be validated for rapid, multiplexed detection of RTI pathogens and AMR markers using advanced characterization techniques such as TEM, XRD, UV-Vis spectroscopy, light scattering, and microscopy. Impact and Significance: The developed diagnostic platform will offer unprecedented sensitivity and speed for point-of-care detection of respiratory infections and resistance markers. This technology has the potential to reduce inappropriate antibiotic use, support NHS diagnostic capacity, and enhance AMR surveillance, especially in community and resource-limited settings due to its low-cost, power-free design. Training and Development: The student will receive interdisciplinary training in nanoparticle synthesis and characterization, colloid and interface science, microfluidics, lateral flow assay engineering, translational diagnostics, AMR-focused assay development, digital image analysis, and Python-based data processing. Opportunities for scientific publishing, international conference presentations, and professional development are provided through the supervisors’ extensive academic and industry networks. Research Environment: The project is jointly hosted by the Bolognesi and Nguyen labs, offering a vibrant, interdisciplinary, and supportive environment. Students benefit from regular one-to-one supervision, group meetings, and access to state-of-the-art analytical and fabrication facilities at UCL Chemistry and the Royal Institution. Both supervisors are active in national and international scientific communities, providing excellent networking and career development opportunities. Eligibility and Requirements: Applicants should have a background in chemistry, nanomaterials, biomedical engineering, or related disciplines. Experience with nanoparticle synthesis, microfluidics, or diagnostic assay development is advantageous. Interest in translational diagnostics and AMR is desirable. Strong academic credentials and relevant laboratory skills are expected. Funding: The studentship provides a stipend at the UKRI rate and is expected to cover tuition fees as per UKRI norms. Application Process: Applications should be submitted via the Centre for Doctoral Training in Engineering Solutions for Antimicrobial Resistance. The deadline for submission is 12 January 2026. For further details and instructions, visit the UCL application link provided.