This PhD project, hosted at the Institute of Bioorganic Chemistry, Polish Academy of Sciences in Poland, focuses on advancing single-molecule RNA sequencing and structure analysis for custom genomic regions. The research addresses the limitations of conventional RNA sequencing, which often fails to capture the full diversity of RNA species, particularly those with complex structures, low abundance, or extensive alternative splicing. The project aims to develop an integrated strategy combining targeted enrichment, adaptive sampling, and structural analysis to achieve precise single-molecule resolution of alternative splicing, RNA folding, and chemical modifications. These methods will be applied to study long noncoding RNAs and detect cancer biomarkers in liquid biopsy samples, with broad applications in transcriptomics, cancer diagnostics, and RNA therapeutics.

The successful candidate will join the cobRNA Lab, a vibrant and multidisciplinary team dedicated to developing innovative genomic and bioinformatic approaches for the identification and functional characterization of long noncoding RNAs across vertebrate genomes. The lab fosters a collaborative and inclusive environment, actively participating in international initiatives such as GENCODE and organizing scientific events like the EMBO Workshop “Non Coding RNA Medicine.” The project includes secondments to collaborating groups in Ireland and Spain, requiring a willingness to travel and spend time abroad. Applicants must hold a Master’s degree in Biology, Biotechnology, or a related field, and must not already possess a doctoral degree.

Required skills include basic knowledge of RNA sequencing, experience with human cell line culture, and proficiency in English. Familiarity with Linux and bioinformatics is desirable. The position is funded as part of the INT2ACT Doctoral Network, with details on stipend and benefits available via the recruitment web page. The application deadline is January 31, 2026.

For more information, candidates can contact Prof. Barbara Uszczyńska-Ratajczak or visit the INT2ACT website.