Bioengineering a Dynamic Culture Platform to Improve the Maturity of iPSC-Derived Skeletal Muscle

This PhD project at the Murdoch Children’s Research Institute (MCRI) focuses on bioengineering a dynamic culture platform to enhance the maturity of skeletal muscle tissues derived from human induced pluripotent stem cells (iPSCs). Musculoskeletal disorders can cause significant disability and impact quality of life, and this research aims to advance understanding of disease mechanisms and support the development of future therapies. The project leverages a skeletal microtissue platform, with ongoing research into tissue maturation using 3D culture, electrical stimulation, and metabolic substrate control. The core objective is to apply bioengineering techniques to create a culture system capable of controlling mechanical stretch and relaxation, thereby improving the maturation of stem cell-derived muscle tissues. The research will involve human pluripotent stem cell culture, directed differentiation protocols, soft lithography, 3D printing, high throughput confocal microscopy, and molecular biology methods. The successful candidate will work within the Stem Cell Biology Department, which houses over 200 researchers dedicated to developing stem cell-based therapies for currently incurable diseases. MCRI offers state-of-the-art facilities for iPSC derivation, gene editing, and drug screening, providing an excellent environment for cutting-edge research. Applicants should have a strong background in biomedical sciences, biomedical engineering, or cardiovascular biology, and hold an Honours or Masters degree or equivalent work experience. English language proficiency is required for students enrolled through the University of Melbourne. Eligibility and competitiveness for a PhD scholarship is desirable, and more information on scholarships is available via the University of Melbourne graduate research scholarships page. To apply, candidates should send a detailed CV, names and contact information of two referees, copies of transcripts, a cover letter highlighting research experience and capabilities, and evidence of English proficiency (if applicable) to Richard Mills ([email protected]). The application deadline is April 28, 2026. References supporting the research environment and project include recent publications in Biomaterials, Cell Reports, and Cell, highlighting the department’s expertise in developing human skeletal muscle platforms and cardiac organoids. This opportunity is ideal for candidates passionate about stem cell biology, tissue engineering, and translational biomedical research, offering access to world-class facilities and a collaborative research community at one of the leading child health institutes globally.