This funded PhD position at the University of Udine, Italy, focuses on investigating the role of Cyclophilin D (CypD), a mitochondrial matrix protein, in mitochondrial RNA (mtRNA) metabolism. While CypD is traditionally known for its role in regulating the mitochondrial permeability transition pore (mPTP), recent findings from Dr. Carlo Vascotto’s laboratory suggest it may also be involved in post-transcriptional regulation of mitochondrial gene expression.

The project aims to elucidate the functional interactions between CypD and mtRNAs, potentially revealing new mechanisms in mitochondrial biology and identifying therapeutic targets for diseases linked to mitochondrial dysfunction. The research will involve developing a novel protocol for immunoprecipitating CypD–RNA complexes from mitochondrial fractions, followed by high-throughput RNA sequencing to identify and classify bound mtRNA species.

Bioinformatic analyses will characterize RNA families and regulatory motifs associated with CypD binding. Functional relevance will be assessed by modulating CypD expression in cell models using shRNA knockdown and overexpression systems, with subsequent analyses including qPCR, Western blotting, and mitochondrial functional assays to measure changes in mtRNA abundance, stability, and organelle physiology. The host laboratory, led by Dr. Vascotto, specializes in DNA repair mechanisms, mitochondrial RNA processes, and the role of mitochondria in tumor progression and resistance.

The lab is well-equipped with facilities for mammalian cell culture, flow cytometry, advanced microscopy, and instruments for monitoring cell viability, apoptosis, and mitochondrial respiration. The project includes secondments to collaborating groups in Poland (Prof. Barbara Uszczynska-Ratajczak, Institute of Bioorganic Chemistry, Polish Academy of Sciences) and the UK (Dr. Alexander Jackson, Nanovery Ltd.), so candidates must be willing to travel and spend time abroad.

Eligibility requires a Master’s degree in Biology, Biotechnology, or a related field, and compliance with the mobility rule (not residing in Italy for more than 12 months in the past 36 months). Research experience in cellular and molecular biology is required, with mitochondrial and/or RNA biology experience as a strong advantage. Proficiency in English is mandatory, demonstrated by a recognized test unless exempted.

Applicants must submit a statement of interest, CV, university examination certificate with marks, and a final degree certificate translated into English (if not available, can be submitted later), all merged into a single PDF. Selected candidates will be invited for an interview and must provide referee contact details. The application deadline is January 31, 2026.

For more information, visit the INT2ACT recruitment page or contact Dr. Carlo Vascotto.