Making and Breaking Myosin Filaments: How Smooth Muscle Cells Modulate Their Contractile Behaviour University of Leeds in United Kingdom

This PhD project, based at the University of Leeds and funded by the BBSRC Yorkshire Bioscience Doctoral Training Partnership (YBDTP), investigates the structure and function of contractile proteins in smooth muscle cells—a critical component in processes such as blood pressure regulation and gastrointestinal motility. Despite their importance, the molecular organization and remodeling of the contractile cytoskeleton in smooth muscle cells remain poorly understood, with implications for diseases like aortic aneurysms and other chronic conditions. The project will employ a multidisciplinary approach, utilizing cutting-edge techniques such as cryo-electron tomography to visualize the contractile cytoskeleton in primary smooth muscle cells and tissues at unprecedented resolution.

The research will leverage advanced facilities at Leeds, including the new Cryo-FIB SEM and state-of-the-art cryo-microscopes. Additional methodologies include advanced labelling strategies, live cell imaging to observe cytoskeletal remodeling in response to pharmacological treatments, and the use of Turbo-ID and mass spectrometry to identify novel protein interactions. Super-resolution imaging will be used to pinpoint the precise locations of these proteins within the cytoskeleton.

The project is supervised by Professor Michelle Peckham, Dr. Charlie Scarff, and Dr. K Riches-Suman, who are leading experts in structural and cell biology. The YBDTP provides a vibrant, interdisciplinary training environment across several universities in the Yorkshire and Teesside region, offering doctoral researchers access to a broad range of research skills in biosciences, biotechnology, and data analysis, as well as professional development for both academic and non-academic careers.

The studentship is fully funded for four years, covering tuition fees at the UK rate, research costs, and a tax-free stipend at the UKRI minimum rate (£20,780 for 2025/26 entry). A limited number of full studentships are available for exceptional international applicants, though they must cover their own visa, NHS surcharge, and travel costs. Applicants should have or expect to obtain at least an upper second-class honours degree (or equivalent) in a relevant biological, chemical, or physical science or mathematics.

The program values diversity and encourages applications from underrepresented groups. The application deadline is 7 January 2026, and candidates may apply for up to two YBDTP projects. Shortlisted applicants will be invited for interview in February 2025.

For further information, prospective students can contact the supervisors or the YBDTP office.