PhD Studentship: Biochemical Mechanisms of Rif1 and Protein Phosphatase 1 in DNA Replication Initiation University of Aberdeen in United Kingdom

This PhD studentship at the University of Aberdeen offers an exciting opportunity to investigate the biochemical mechanisms regulating DNA replication initiation, a process fundamental to all life and a key target for cancer therapeutics. The project focuses on the protein Rif1, a major regulator of DNA replication, which controls replication by directing Protein Phosphatase 1 (PP1) to dephosphorylate the DNA replication machinery, counteracting kinases that activate replication origins. Understanding how Rif1 and PP1 interact to regulate the timing of replication origin activation is central to this research.

Using biochemical and molecular genetic approaches, the student will dissect the molecular mechanism through which Rif1 controls PP1 catalytic activity and explore how the Rif1-PP1 complex is recruited to origin sites via interactions with replication proteins. The research will begin in yeast, where replication initiation is best understood, and later extend to human replication systems, aiming to elucidate how the eukaryotic replication timing programme is determined. Since Rif1 also regulates chromosome maintenance processes such as DNA repair, nascent DNA protection, and 3D chromosome organisation, the findings will have broad implications for understanding mechanisms that ensure chromosome stability.

The successful candidate will join a vibrant, well-funded research team within the Institute of Medical Sciences at the University of Aberdeen, collaborating with world-leading laboratories including Joe Yeeles (MRC Laboratory of Molecular Biology) and Rebecca Page (University of Connecticut). The Institute provides access to advanced facilities such as Microscopy, Flow Cytometry, Genomics, and Proteomics, and the student will benefit from training in yeast molecular genetics and biochemical analyses, contributing to research relevant to cancer and genome instability disorders.

Applicants should have or be about to obtain a Bachelors or Masters degree at least at Upper Second level (2:1 UK Honours or equivalent), with laboratory research experience in biochemistry, structural biology, genetics, or molecular biology. The University encourages applications from diverse backgrounds and those returning from career breaks or industry. International candidates are welcome but must fund the difference between UK and international tuition fees.

Funding for this 4-year studentship is provided by the University of Aberdeen, alongside a Wellcome Discovery Award. The studentship covers a stipend (£20,780 per annum) and University fees at the Home/UK rate. Full funding is available to Home/UK students only; international applicants must fund the difference between home and international fees (£22,300 per annum).

To apply, send your complete application as a single email to [email protected] by 23:59 GMT on 31st March 2026, with the subject line: 'Anne Donaldson PhD Project - [Your Name]'. Include a cover letter addressed to the supervisor, an up-to-date CV, and clear copies of degree certificates and transcripts. No research proposal or additional documents are required. For informal enquiries, contact Professor Anne Donaldson ([email protected]) or Dr Shin-ichiro Hiraga ([email protected]).