PhD Studentship – CRISPR Kinome Screen to Identify Synthetic Lethality Targets in Meningioma University of Plymouth in United Kingdom

[Full Home tuition fees plus a stipend of £19,215 per annum (2025-26 rate; 2026-27 rate TBC) for 4 years. International applicants must cover the difference between Home and International fees. No additional funding for NHS Immigration Health Surcharge, visa costs, or flights.]

This fully funded four-year PhD studentship at the University of Plymouth offers an exciting opportunity to advance research in translational neuro-oncology. The project focuses on meningiomas, the most common primary brain tumours, and aims to identify novel synthetic lethality targets using a high-throughput microfluidic CRISPR kinome screening platform. The research will leverage the Brunello kinome library to introduce CRISPR sgRNAs into primary meningioma cells, followed by exposure to the drug brigatinib. Using state-of-the-art magnetic ranking cytometry and microfluidic sorting, the project will quantify phospho-ERK levels to isolate resistant and sensitized cell populations. Subsequent RNA sequencing and bioinformatic analyses will identify top candidate genes for synthetic lethality, which will be validated through RNAi and pharmacological approaches, with an emphasis on drug repurposing for rapid clinical translation.

The successful candidate will join a collaborative, interdisciplinary team and benefit from access to a molecularly annotated biobank of primary meningioma tissues and established in vivo models. The project timeline includes overlapping work packages in CRISPR library screening, microfluidic sorting, single-cell sequencing, and functional validation. Comprehensive training will be provided in molecular biology, high-throughput microfluidics, CRISPR gene editing, single-cell transcriptomics, and preclinical validation, equipping the student with advanced skills for a career in biomedical research.

Applicants should hold a first or upper second class honours degree in a relevant subject or a related Masters qualification. Non-native English speakers must demonstrate proficiency with an IELTS Academic score of 6.5 (minimum 5.5 in each component) or equivalent. The studentship covers full Home tuition fees and a stipend of £19,215 per annum (2025-26 rate; 2026-27 rate TBC) for four years. International applicants are welcome but must cover the difference between Home and International tuition fees. Additional costs such as NHS Immigration Health Surcharge, visa fees, and travel are not covered. Full-time employment is not permitted during the studentship.

To apply, submit your application via the University of Plymouth portal, including a CV, personal statement, degree certificates and transcripts, and contact details for two academic referees. Shortlisted candidates will be invited for interview around the week commencing 20th April 2026. For informal enquiries, contact Dr Liyam Laraba at [email protected].