PhD in Vasculature-on-chip Models for Improving Nanobubble-Enhanced Ultrasound Imaging

This PhD position at Eindhoven University of Technology focuses on developing advanced vasculature-on-chip models to improve nanobubble-enhanced ultrasound imaging for cancer diagnostics. The project addresses the limitations of current imaging techniques in detecting and diagnosing cancers, particularly prostate cancer, by leveraging the unique properties of nanobubbles and microfluidic technology. The research is part of a larger collaborative effort involving two PhD students and a postdoc at Eindhoven University of Technology and the University of Twente, with additional industrial and clinical partners. The successful candidate will design, fabricate, and experimentally characterize in-vitro vasculature-on-chip models. These microfluidic chips will replicate the complex geometry and biology of tumor microvasculature, including 3D networks of perfusable lumens, endothelialization, and integration of tumor cells within a relevant extracellular matrix. The chips must be acoustically transparent for ultrasound transmission and ideally optically transparent for validation via optical imaging. The project utilizes innovative 3D printing techniques to create sacrificial templates embedded in hydrogels, which are then seeded with endothelial cells to form biologically relevant vessel networks. Further advancements will include modulating and characterizing endothelial permeability using inflammatory agents and fluorescent microscopy, providing models with varying permeability for nanobubble ultrasound experiments. The PhD student will be embedded in the Microsystems research section of the Department of Mechanical Engineering, supervised by Professor Jaap den Toonder. The Microsystems group is renowned for its expertise in microfabrication, microfluidics, and organ-on-chip research, supported by the state-of-the-art Microfab/lab facility. The project is highly interdisciplinary, combining bubble physics, microfluidics, ultrasound modeling, and signal analysis to create a comprehensive framework for improved cancer imaging. Applicants should have a background in mechanical or biomedical engineering, with experience in microfluidics, microfabrication, organ-on-chip, cell culture, and cell/tissue characterization preferred. The position offers full-time employment for four years, competitive salary and benefits, and opportunities for professional development within an international and collaborative academic environment. The university provides excellent infrastructure, training programs, and support for international candidates, including a tax compensation scheme and staff immigration assistance. Applications must be submitted online and include a cover letter, CV, and contact information for three references. The vacancy will remain open until filled, with priority given to complete applications. For further information, contact Professor Jaap den Toonder at [email protected] or HR Advisor Femke Verheggen at [email protected].