Time-Resolved CryoEM PhD Project: Dynamic Structural Biology at University of Leicester University of Leicester in United Kingdom

This PhD project at the University of Leicester offers a unique opportunity to develop and apply advanced techniques in time-resolved structural biology, focusing on the frontiers of cryo-electron microscopy (cryoEM). The research aims to unravel how macromolecular complexes assemble and function as molecular machines, a fundamental question in molecular biology. CryoEM enables high-resolution characterization of molecules in various conformational states, but the chronological relationships and functional implications of these states remain largely unexplored.

Time-resolved cryoEM is a cutting-edge approach that allows researchers to analyze dynamic biological processes and understand mechanisms of action at the molecular level. The Midlands Regional CryoEM Facility at the University of Leicester has recently acquired the first commercially available Speed Blot system, which facilitates rapid sample preparation for capturing reaction intermediates on millisecond timescales. This technology will be further developed and applied in the project to investigate the dynamic mechanisms of large molecular machines, with an initial focus on transient intermediate states in DNA-repair machinery.

The project is a collaboration with Dr Amanda Chaplin, an expert in non-homologous end-joining DNA repair, who will be part of the supervisory committee. Additional collaborations with researchers in the Leicester Institute for Structural and Chemical Biology (LISCB) are anticipated, broadening the scope of the research. Students will have direct access to world-class facilities, including a dedicated low humidity laboratory for sample preparation, a ThermoFisher Scientific Titan Krios electron microscope with Gatan K3 direct detector, and a bespoke computational cluster for data analysis.

Throughout the PhD, students will gain extensive experience in cryoEM, encompassing sample preparation, microscope operation, and computational analysis. The project will involve developing time-resolved methodologies for cryoEM sample preparation and collaborating with scientists on a variety of topics to address important, biologically relevant molecular questions.

The LISCB Studentship provides 3.5 years of UK tuition fees and a stipend at UKRI rates (£21,805 per year for 2026/7). International applicants are welcome but must demonstrate their ability to fund the difference between UK and overseas fees (£19,012 per year for 2026/7). Visa, NHS charges, and travel costs must also be met by the student. In exceptional cases, a full overseas fee waiver may be possible, though it is not guaranteed.

Applicants should have a strong background in molecular biology, biochemistry, or a related discipline. Experience with structural biology, electron microscopy, or computational analysis is desirable but not essential. For project enquiries, contact Dr AC Chaplin at [email protected]. To apply, visit the University of Leicester funded opportunities webpage and follow the instructions provided. The application deadline is March 29, 2026.