Klaus Eyer
Associate Professor
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About
Klaus Eyer is an Associate Professor at Aarhus University in Denmark. His research focuses on single-cell analysis, with recent articles exploring topics such as antibody secretion, cytokine release dynamics, and the functional heterogeneity of immune cells. He is particularly interested in the development of innovative microfluidic platforms for studying immune responses and the quantitative assessment of immunization effects.
Recent Grants
Grant: Close
New bridges across the translational gap
Open Date: 2019-09-01
Close Date: 2021-08-01
Grant: Close
High-throughput single-cell phenotypic analysis of functional antibody repertoires
Open Date: 2019-02-01
Close Date: 2024-01-31
Grant: Close
Functional high-throughput single-cell analysis towards the rational development of new vaccines
Open Date: 2016-09-01
Close Date: 2021-09-01
Grant: Close
Time resolved ultrahigh resolution phenotypic mapping of antibody producing cells
Open Date: 2016-09-01
Close Date: 2018-02-28
Grant: Close
Detection of circulating tumor cells in patients
Open Date: 2016-05-01
Close Date:
Positions (2)
Articles (10)
Single‐Cell Profiling Reveals Functional Heterogeneity and Serial Killing in Human Peripheral and Ex Vivo‐Generated CD34+ Progenitor‐Derived Natural Killer Cells
Increasing evidence suggests that natural killer (NK) cells are composed of distinct functional subsets. This multifunctional role has made them an attractive choice for anticancer immunotherapy. A functional NK cell repertoire is generated through cellular education, resulting in a heterogeneous NK cell population with distinct capabilities responding to different stimuli. The application of a high‐throughput droplet‐based microfluidic platform allows monitoring of NK cell‐target cell interactions at the single‐cell level and in real‐time. A variable response of single NK cells toward different target cells is observed, and a distinct population of NK cells (serial killers) capable of inducing multiple target lysis is identified. By assessing the cytotoxic dynamics, it is shown that single umbilical cord blood‐derived CD34+ hematopoietic progenitor (HPC)‐NK cells display superior antitumor cytotoxicity. With an integrated analysis of cytotoxicity and cytokine secretion, it is shown that target cell interactions augment cytotoxic as well as secretory behavior of NK cells. By providing an integrated assessment of NK cell functions by microfluidics, this study paves the way to further functionally characterize NK cells ultimately aimed to improve cancer immunotherapy.
Year:
2022
Collaborators (4)
Jean Baudry
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Paul de Jonge
Senior Scientist & Deputy Head Cell Therapy Facility
Radboudumc
Mark van Turnhout
Eindhoven University of Technology
Jurjen Tel
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