Thomas Wichard

Axenic cultures of the green seaweed Ulva mutabilis were inoculated with bacteria providing essential algal growth and morphogenesis-promoting factors (AGMPFs) and were exposed to temperature shifts from 18 °C to 30 °C. The temperature-dependent effect of bacteria on longitudinal algal growth and the molecular composition of the chemosphere in the algal culture medium was explored. The reductionist tripartite model system of U. mutabilis , Roseovarius sp. MS2, and Maribacter sp. MS6 was applied as a reference and has been changed by substituting Roseovarius with isolates that phenocopy this strain. Rathayibacter festucae IH2 and Roseovarius aestuarii G8 boosted growth at 18 °C but slowed it down at 30 °C. Additional inoculation of Roseovarius sp. MS2 mitigated these adverse bacterial effects partially. At 30 °C, the molecular profile of the chemosphere differed dramatically between all tested tripartite communities, indicating different traits of the same bacterium with changing temperatures. Functional examinations should, therefore, accompany microbiome analysis to detect changing traits with the same microbiome composition.

Microbes can modify their hosts' stress tolerance, thus potentially enhancing their ecological range. An example of such interactions is Ectocarpus subulatus , one of the few freshwater‐tolerant brown algae. This tolerance is partially due to its (un)cultivated microbiome. We investigated this phenomenon by modifying the microbiome of laboratory‐grown E. subulatus using mild antibiotic treatments, which affected its ability to grow in low salinity. Low salinity acclimation of these algal‐bacterial associations was then compared. Salinity significantly impacted bacterial and viral gene expression, albeit in different ways across algal‐bacterial communities. In contrast, gene expression of the host and metabolite profiles were affected almost exclusively in the freshwater‐intolerant algal‐bacterial communities. We found no evidence of bacterial protein production that would directly improve algal stress tolerance. However, vitamin K synthesis is one possible bacterial service missing specifically in freshwater‐intolerant cultures in low salinity. In this condition, we also observed a relative increase in bacterial transcriptomic activity and the induction of microbial genes involved in the biosynthesis of the autoinducer AI‐1, a quorum‐sensing regulator. This could have resulted in dysbiosis by causing a shift in bacterial behaviour in the intolerant algal‐bacterial community. Together, these results provide two promising hypotheses to be examined by future targeted experiments. Although they apply only to the specific study system, they offer an example of how bacteria may impact their host's stress response.