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Francisca Lottersberger

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Associate Professor

Linköping University
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Sweden

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Linköping University

Linköping University

PhD Student Position in Medical Science (Molecular Biology, Biotechnology) at Linköping University

Linköping University in Sweden is inviting applications for a PhD student position in Medical Science, with a research focus on molecular biology, biotechnology, and telomere biology. The successful candidate will join the Division of Molecular Medicine and Virology (MMV) at the Department of Biomedical and Clinical Sciences (BKV). The research project aims to understand the consequences of telomere deprotection in normal and malignant cells, investigating both cellular and molecular mechanisms, including DNA end processing, chromatin dynamics, and repair outcomes. Experimental work will involve cell culture, genetic manipulations, genomic rearrangement analysis, telomere assays, and RNA sequencing, as well as computational bioinformatics analysis. Applicants must hold a Master’s degree in Molecular Biology, Biotechnology, or a related field, or have completed at least 240 credits (with at least 60 in advanced courses), or possess equivalent knowledge. Candidates expecting to graduate before September 2026 are also eligible. Required skills include proficiency in English, strong communication and teamwork abilities, and a solid background in molecular techniques and biomedical research. Experience in genome stability, DNA damage response, telomere biology, mammalian cell culture, transient transfection, viral transduction, plasmid cloning, immunoblot, immunofluorescence, FISH, metaphase analysis, and sgRNA design is highly desirable. The PhD position is fully salaried, with employment benefits and salary progression according to university policy. The appointment is for four years full-time, initially for one year and renewable for up to five years depending on progress and departmental duties. The application deadline is October 3, 2026. Applications must include a motivation letter and CV, and should be submitted via the Linköping University job portal. For further information, contact Associate Professor Francisca Lottersberger or Annelie Lindström, Director of Third Cycle studies at BKV. This is an excellent opportunity for candidates with a strong interest in medical science, molecular biology, and biotechnology to advance their research careers at a leading European university. The university values diversity and equal opportunities in its recruitment process.

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Articles (1)

DNA-PK controls Apollo’s access to leading-end telomeres

The complex formed by Ku70/80 and DNA-PKcs (DNA-PK) promotes the synapsis and the joining of double strand breaks (DSBs) during canonical non-homologous end joining (c-NHEJ). In c-NHEJ during V(D)J recombination, DNA-PK promotes the processing of the ends and the opening of the DNA hairpins by recruiting and/or activating the nuclease Artemis/DCLRE1C/SNM1C. Paradoxically, DNA-PK is also required to prevent the fusions of newly replicated leading-end telomeres. Here, we describe the role for DNA-PK in controlling Apollo/DCLRE1B/SNM1B, the nuclease that resects leading-end telomeres. We show that the telomeric function of Apollo requires DNA-PKcs’s kinase activity and the binding of Apollo to DNA-PK. Furthermore, AlphaFold-Multimer predicts that Apollo’s nuclease domain has extensive additional interactions with DNA-PKcs, and comparison to the cryo-EM structure of Artemis bound to DNA-PK phosphorylated on the ABCDE/Thr2609 cluster suggests that DNA-PK can similarly grant Apollo access to the DNA end. In agreement, the telomeric function of DNA-PK requires the ABCDE/Thr2609 cluster. These data reveal that resection of leading-end telomeres is regulated by DNA-PK through its binding to Apollo and its (auto)phosphorylation-dependent positioning of Apollo at the DNA end, analogous but not identical to DNA-PK dependent regulation of Artemis at hairpins.

Year:

2024

Collaborators (2)

Titia de Lange

Rockefeller University

UNITED STATES

Patrik Eickhoff

Institute of Cancer Research

UNITED KINGDOM
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