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Dafne Campigli Di Giammartino

1 year ago

PhD Position: Targeted Demethylation of ncRNAs Involved in 3D Chromatin Hubs in Cancer University of Udine in Italy

Degree Level

PhD

Field of study

Biotechnology

Funding

Funded PhD Project (Students Worldwide)

Deadline

Expired

Country flag

Country

Italy

University

University of Udine

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Keywords

Biotechnology
Biology
Stem Cell Biology
Health Science
Transcriptional Regulation
Chromatin Biology
Rna-seq
Epigenetic
Glioblastoma
Genomic
Non-coding Rna
C
Translation Biology
Crispr/cas9

About this position

This PhD project investigates the role of targeted demethylation of non-coding RNAs (ncRNAs) in the regulation of 3D chromatin architecture within cancer stem cells. The research focuses on long non-coding RNAs (lncRNAs) and their epitranscriptomic modifications, particularly N6-methyladenosine (m6A), which are known to influence chromatin dynamics and gene expression. The project aims to implement a dCas13 protein fused with an m6A eraser to reverse m6A modifications on selected ncRNAs enriched in highly connected chromatin regions.

The work is structured in three phases: establishing a dox-inducible system for dCas13-m6Aeraser expression in patient-derived glioma stem cell lines, validating efficient m6A removal from candidate ncRNAs, and evaluating the effects on 3D chromatin interactivity using advanced techniques such as Hi-C, HiChIP, and 4C-seq. These analyses will be correlated with changes in gene expression (RT-qPCR, RNA-seq) and the epigenetic landscape (ChIP-seq, Cut&Tag). The overarching goal is to uncover novel molecular mechanisms by which ncRNAs and their modifications shape chromatin architecture and regulate gene expression, potentially identifying new therapeutic targets for cancer treatment. The host laboratory, led by Dr.

Dafne Campigli Di Giammartino at the Istituto Italiano di Tecnologia, is part of the IIT RNA Flagship program and collaborates with international partners, including the Human Technopole Functional Genomics unit and the FANTOM6 project. The position offers strong collaborative opportunities, with expected secondments to laboratories in Poland and Ireland.

Applicants must have a Master’s degree in a relevant field, research experience in cellular and molecular biology, and proficiency in English. Funding is provided through the INT2ACT Doctoral Network, including a stipend and support for international secondments. The application deadline is January 31, 2026.

Funding details

Funded PhD Project (Students Worldwide)

What's required

Applicants must hold a Master’s degree in Biology, Molecular Biology, Biotechnology or a related program. Candidates must not already possess a doctoral degree. The mobility rule applies: researchers must not have resided or carried out their main activity in Italy for more than 12 months in the 36 months immediately before recruitment. Research experience in cellular and molecular biology techniques is required. Familiarity with non-coding RNA, CRISPR technologies, and/or previous experience in producing NGS data (such as ChIP-seq, RNA-seq) is a strong advantage. Proficiency in English is required, demonstrated by an English test (IELTS, TOEFL, Cambridge English) unless exempted by nationality or prior English-taught degree. Good oral and written communication skills are essential.

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Professors

Dr. at INT2ACT

University Name
Country flag

Italy

Research Interests

C

Health Science

Glioblastoma

Rna-seq

Chromatin Biology

Dr. at INT2ACT

University Name
Country flag

Italy

Research Interests

Health Science

Translation Biology

Rna-seq

Biology

Crispr/cas9

Dr. at INT2ACT

University Name
Country flag

Italy

Research Interests

Health Science

Biology

Translation Biology

Rna-seq

Rna Biology

Professor at INT2ACT

University Name
Country flag

Italy

Research Interests

Tissue Engineering

Cell Biology

Translation Biology

Biology

Health Science