Alexander Yakunin

Associate Professor

University of Toronto

Canada

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Dr. Alexander Yakunin is an Associate Professor at the University of Toronto, Canada. His research encompasses various areas including chemoenzymatic reactions, structural biology of glycosidases and peroxidases, and the development of sustainable biotechnological processes. Recent publications highlight his work on the thermal stability of enzymes, CRISPR technology in E. coli, and the biochemical properties of lignin-oxidizing bacteria.

Articles (14)

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2025

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2025

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2025

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2024

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2024

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2024

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2024

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2020

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2020

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2020

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2020

Collaborators (6)

Emil Pai

Professor emeritus

University of Toronto

CANADA

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Peter Golyshin

Bangor University

UNITED KINGDOM

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Radhakrishnan Mahadevan

University of Toronto

CANADA

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Robert Flick

University of Toronto

CANADA

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Kosuke Fujishima

Tokyo Institute of Technology

JAPAN

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Elizabeth Edwards

Professor

University of Toronto

CANADA

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Alexander Yakunin

Research Interests

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Articles (14)

Methanol-Driven, One-Pot Chemoenzymatic S-Adenosylmethionine Regeneration for Ambient Cobalamin-Dependent Methyltransferase Reactions

Structural and functional insights into extreme thermal stability and activity of two GH12 domains of a multidomain glycosidase from a hyperthermophilic euryarchaeon

Structural and Biochemical Insights into Lignin-Oxidizing Activity of Bacterial Peroxidases against Soluble Substrates and Kraft Lignin

Ni(II)-binding affinity of CcNikZ-II and its homologs: the role of the HH-prong and variable loop revealed by structural and mutational studies

Biochemical plasticity of the Escherichia coliCRISPR Cascade revealed by in vitro reconstitution of Cascade activities from purified Cas proteins

A random mutagenesis screen enriched for missense mutations in bacterial effector proteins

Amino Acid Self-Regenerating Cell-Free Protein Synthesis System that Feeds on PLA Plastics, CO2, Ammonium, and α-Ketoglutarate

One-pot chemo-enzymatic synthesis and one-step recovery of length-variable long-chain polyphosphates from microalgal biomass

Defluorination Capability of l‐2‐Haloacid Dehalogenases in the HAD‐Like Hydrolase Superfamily Correlates with Active Site Compactness

Lignin-oxidizing activity of bacterial laccases characterized using soluble substrates and polymeric lignin

A Bifunctional Polyphosphate Kinase Driving the Regeneration of Nucleoside Triphosphate and Reconstituted Cell-Free Protein Synthesis

Biocatalytic in Vitro and in Vivo FMN Prenylation and (De)carboxylase Activation

One-Pot Biocatalytic Transformation of Adipic Acid to 6-Aminocaproic Acid and 1,6-Hexamethylenediamine Using Carboxylic Acid Reductases and Transaminases

Rational engineering of 2-deoxyribose-5-phosphate aldolases for the biosynthesis of (R)-1,3-butanediol

Collaborators (6)

Emil Pai

Peter Golyshin

Radhakrishnan Mahadevan

Robert Flick

Kosuke Fujishima

Elizabeth Edwards

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