Chang-Jun Cha

A Gram-stain-negative, aerobic and motile bacterial strain, designated CJ34 T , was isolated from Han River water in the Republic of Korea. Strain CJ34 T grew optimally on tryptic soy agar at 30 °C and pH 7.0 in the absence of NaCl. Results of phylogenetic analysis based on 16S rRNA gene sequence showed that strain CJ34 T belonged to the genus Comamonas within the family Comamonadaceae and was most closely related to Comamonas testosteroni ATCC 11996 T and Comamonas thiooxydans DSM 17888 T (both 98.63 % similarity). The average nucleotide identity values between strain CJ34 T and two closely related type strains C. testosteroni ATCC 11996 T and C. thiooxydans DSM 17888 T were 82.77 and 82.73 %, respectively. The major isoprenoid quinone of strain CJ34 T was ubiquinone Q-8. The major cellular fatty acids of strain CJ34 T were C 16 : 0 , C 16 : 1 ω 6 c and/or C 16 : 1 ω 7c and C 18 : 1 ω 6 c and/or C 18 : 1 ω 7 c . The predominant polar lipids of strain CJ34 T were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified aminophospholipid. Whole genome sequencing revealed that strain CJ34 T had a genome of 4.9 Mbp and the G+C content of the genomic DNA was 59.73 mol%. On the basis of the results of this polyphasic taxonomy study, strain CJ34 T represents a novel species in the genus