Dalibor Broznić

Ticks are significant vectors of pathogens in human and veterinary medicine and have been identified as (re)emerging health threats. The primary objective of this study was to collect new data on the fauna of hard ticks within the region of Istria with a focus on spatial distribution using a geographical information system (GIS). All tick specimens were collected over three years (2020–2023), and this research included all 41 self-government units of Istria and Brijuni Islands National Park. Ticks were collected using the flagging/dragging method and manually from hosts (humans, domestic, or wild animals). In addition, morphological identification using tick keys was performed. The obtained data were used to create maps and feed models and to predict risk assessments. Collected data reveal the predominant presence of Ixodes ricinus, accounting for (n = 446) or 48.1% of the tick population. Rhipicephalus sanguineus (Ixodida: Ixodidae) follows with (n = 253) or 27.23%, and Hyalomma marginatum represents (n = 136) or 14.64% of the tick species collected using the host method in the region. Tick–host relationships are complex and influenced by a range of ecological and environmental factors. The results of this research will contribute to a better understanding, identification, and prediction of the changes in their geographic ranges and help in the prevention and control of zoonosis transmitted to humans by ticks. The obtained results mapped using GIS support the first study on the spatial distribution of ticks in the region of Istria in Croatia.

In January 2023, the derogation loophole was closed on “emergency authorisations” for the use of three out of five neonicotinoids in all EU states. In this study, we analysed the sorption/desorption behaviour and kinetic parameters of acetamiprid and thiacloprid, the two neonicotinoids that are still approved for use, either regularly or under emergency authorisations in the EU, and widely used worldwide. Sorption and desorption curves in four soils with different organic matter content were analysed using four kinetic models, namely, Lagergren’s pseudo first-order model, two-site model (TSM), Weber–Morris intraparticle diffusion model and Elovich’s model. Kinetic parameters were correlated to soil physico-chemical characteristics. To determine the mutual influence of soil characteristics and sorption/desorption parameters in the analysed soils, a factor analysis based on principal component analysis (PCA) was performed. Even though the two insecticides are very similar in size and chemical structure, the results showed different sorption/desorption kinetics. The model that best fits the experimental data was TSM. Thiacloprid showed a more rapid sorption compared to acetamiprid, and, in all soils, a higher proportion sorbed at equilibrium. Intra-particle diffusion seemed to be a relevant process in acetamiprid sorption, but not for thiacloprid. Desorption results showed that acetamiprid is more easily and more thoroughly desorbed than thiacloprid, in all soils. The kinetic behaviour differences stem from variations in molecular structure, causing disparate water solubility, lipophilicity, and acid–base properties.