Gerrit T. S. Beemster

Maize ( Zea mays ), a cold‐sensitive crop, requires cold tolerance for extending the length of the growing season in temperate climates. However, response curves to different cold temperatures and exposure durations are lacking. We used a meta‐analysis approach using data from literature to investigate the effect of cold stress in the maize leaf. We constructed response curves to temperature and exposure durations for 18 key parameters related to leaf growth, photosynthesis, oxidative stress, antioxidants, and the phytohormone ABA. To determine their relevance for cold tolerance, we compared cold tolerant Flint and cold sensitive Dent lines. Treatment temperatures ranged from −20°C to 20°C for cold and from 12°C to 30°C for control and exposure duration from 3 min to 60 days. We found interacting effects of temperature and exposure durations on different response parameters. The strongest difference between Flint and Dent was observed for electrolyte leakage (EL). Our results show that the commonly used 4°C for cold and 25°C for control with medium cold exposure (1−7 days) induces a 50% decrease in shoot dry weight and leaf area and that EL is an easy and reliable indicator for cold tolerance studies.