In-Young Choi

Type 2 diabetes (T2D) is a complex chronic metabolic disorder characterized by hyperglycemia because of insulin resistance. Diabetes with chronic hyperglycemia may alter brain metabolism, including brain glucose and neurotransmitter levels; however, detailed, longitudinal studies of metabolic alterations in T2D are lacking. To shed insight, here, we characterized the consequences of poorly controlled hyperglycemia on neurochemical profiles that reflect metabolic alterations of the brain in both humans and animal models of T2D. Using in vivo 1 H magnetic resonance spectroscopy, we quantified 12 metabolites cross‐sectionally in T2D patients and 20 metabolites longitudinally in T2D db / db mice versus db + controls. We found significantly elevated brain glucose (91%, p < 0.001), taurine (22%, p = 0.02), glucose+taurine (56%, p < 0.001), myo ‐inositol (12%, p = 0.02), and choline‐containing compounds (10%, p = 0.01) in T2D patients versus age‐ and sex‐matched controls, findings consistent with measures in T2D db / db versus control db + littermates. In mice, hippocampal and striatal neurochemical alterations in brain glucose, ascorbate, creatine, phosphocreatine, γ‐aminobutyric acid, glutamate, glutamine, glutathione, glycerophosphoryl‐choline, lactate, myo ‐inositol, and taurine persisted in db / db mice with chronic disease progression from 16 to 48 weeks of age, which were distinct from control db + mice. Overall, our study demonstrates the utility of 1 H magnetic resonance spectroscopy as a non‐invasive tool for characterizing and monitoring brain metabolic changes with T2D progression. image