Keith A. Choate

We report two cases of cutaneous mpox virus infection (formerly known as monkeypox), one of which presented with unusual clinical findings, and demonstrate their histopathological, RNA in situ hybridization (ISH) and ultrastructural characteristics. We observed in two patients with polymerase chain reaction-confirmed mpox virus infection squamous syringometaplasia of the eccrine ducts, a finding that can aid in recognizing this disease and is explained by the demonstration of viral mRNA within eccrine epithelium by ISH.

Basaloid follicular hamartomas (BFH) are benign small basaloid skin tumors that can present as solitary or multiple lesions. Congenital BFH lesions arranged in a segmental distribution have been described, suggesting they derive from a somatic post‐zygotic mutational event. Previously, BFH were described in Happle‐Tinschert syndrome, which results from a post‐zygotic SMO variant and is characterized by segmental BFH with variable involvement of the teeth, skeleton, and central nervous system. Here, we describe two patients with isolated segmental BFH and no systemic involvement. Paired whole exome sequencing of BFH and normal tissue revealed a pathogenic SMO c.1234 C>T, p.L412F variant restricted to BFH tissue. We characterized the proliferation index and expression of Hedgehog and Wnt/beta‐catenin pathway related proteins in segmental BFH compared to sporadic basal cell carcinomas (BCCs) and found that segmental BFH had a lower proliferation index. Although segmental BFH expressed a similar level of Gli‐1 compared to BCCs, levels of LEF‐1 and SOX‐9 expression in BFH were weaker for both and patchier for LEF‐1. Our results show that a somatic SMO activating variant causes segmental BFH. Since these patients are prone to developing BCCs, differences in SOX9, LEF1, and Ki‐67 expression can help distinguish between these two basaloid lesions.