Kiyoshi Takeda

In the intestine, interleukin (IL)‐23 and IL‐22 from immune cells in the lamina propria contribute to maintenance of the gut epithelial barrier through the induction of antimicrobial production and the promotion of epithelial cell proliferation. Several previous studies suggested that some of the functions of the IL‐23/IL‐22 axis on intestinal epithelial cells are shared between the small and large intestines. However, the similarities and differences of the IL‐23/IL‐22 axis on epithelial cells between these two anatomical sites remain unclear. Here, we comprehensively analyzed the gene expression of intestinal epithelial cells in the ileum and colon of germ‐free, Il23 −/− , and Il22 −/− mice by RNA‐sequencing. We found that while the IL‐23/IL‐22 axis is largely dependent on gut microbiota in the small intestine, it is much less dependent on it in the large intestine. In addition, the negative regulation of lipid metabolism in the epithelial cells by IL‐23 and IL‐22 in the small intestine was revealed, whereas the positive regulation of epithelial cell proliferation by IL‐23 and IL‐22 in the large intestine was highlighted. These findings shed light on the intestinal site‐specific role of the IL‐23/IL‐22 axis in maintaining the physiological functions of intestinal epithelial cells.