Maged E. Mohamed

Anethole (AN) is one of the major constituents of several plant oils, demonstrating plentiful pharmacological actions. Ischemic stroke is the main cause of morbidity and death worldwide, particularly since ischemic stroke therapeutic choices are inadequate and limited; thus, the development of new therapeutic options is indispensable. This study was planned to explore the preventive actions of AN in ameliorating cerebral ischemia/reperfusion-induced brain damage and BBB permeability leakage, as well as to explore anethole’s potential mechanisms of action. The proposed mechanisms included modulating JNK and p38 as well as MMP-2 and MMP-9 pathways. Sprague–Dawley male rats were randomly assigned into four groups: sham, middle cerebral artery occlusion (MCAO), AN125 + MCAO, and AN250 + MCAO. Animals in the third and fourth groups were pretreated with AN 125 or 250 mg/kg orally, respectively, for two weeks before performing middle cerebral artery occlusion (MCAO)-induced cerebral ischemic/reperfusion surgery. Animals that experienced cerebral ischemia/reperfusion exhibited amplified infarct volume, Evans blue intensity, brain water content, Fluoro-Jade B-positive cells, severe neurological deficits, and numerous histopathological alterations. MCAO animals exhibited elevated MMP-9 and MMP-2 gene expressions, enzyme activities, augmented JNK, and p38 phosphorylation. On the other hand, pretreatment with AN diminished the infarct volume, Evans blue dye intensity, brain water content, and Fluoro-Jade B-positive cells, improved the neurological score and enhanced histopathological examination. AN effectively lowered MMP-9 and MMP-2 gene expression and enzyme activities and diminished phosphorylated JNK, p38. AN decreased MDA content, amplified GSH/GSSG ratio, SOD, and CAT, decreased the serum and brain tissue homogenate inflammatory cytokines (TNF-α, IL-6, IL-1β), NF-κB, and deterred the apoptotic status. This study revealed the neuroprotective ability of AN against cerebral ischemia/reperfusion in rats. AN boosted blood–brain barrier integrity via modulating MMPs and diminished oxidative stress, inflammation, and apoptosis through the JNK/p38 pathway.

Background: The KSA government has envisioned a national transformation plan via Vision 2030. The purpose of this plan is liberalization which provides an opportunity for the private sector to increase its share in the healthcare system. Evaluating the entrepreneurial potential of healthcare students is essential due to their future role in the healthcare environment. Thus, the goal of the current study is to measure entrepreneurial intentions and identify the characteristics and personality dimensions among junior healthcare students and fresh graduates in Saudi Arabia. Materials and methods: A cross-sectional study design was utilized to survey healthcare students in their final years of study and fresh graduates at King Faisal University (KFU), Saudi Arabia. The research team identified six as the most common entrepreneurial intention traits. Entrepreneurial traits included internal locus of control, innovativeness, risk-taking, proactiveness, autonomy, and problem-solving. A previously recognized and validated questionnaire was distributed through social media platforms and formal university emails. PLS-SEM was employed for the examination of both the measurement and structural models. Results: Internal locus of control was found to have a positive impact on entrepreneurial intention, so H1 was supported. Likewise, innovativeness showed a positive impact on entrepreneurial intention among healthcare students, which confirmed H2. The results also showed that autonomy substantially impacted entrepreneurial intention, so H3 was supported. Similarly, proactiveness has a direct influence on entrepreneurial intention; thus, H5 was supported. Furthermore, problem-solving personality dimensions also had a positive impact on entrepreneurial intention, so H5 and H6 were supported. Conversely, risk-taking propensity exhibited a negative insignificant effect on entrepreneurial intention. Thus, H4 was not supported. Conclusions: Saudi healthcare students revealed their intention towards entrepreneurship and were quite positive about becoming entrepreneurs. Furthermore, students’ entrepreneurial intention was affected by their quality of proactiveness, innovativeness, internal locus of control, problem-solving, and autonomy but not risk-taking propensity.