Mikhail Eltsov

Advances in cryo‐electron microscopy (EM) enable imaging of protein assemblies within mammalian cells in a near native state when samples are preserved by cryogenic vitrification. To accompany this progress, specialized EM labelling protocols must be developed. Gold nanoparticles (AuNPs) of 2 nm are synthesized and functionalized to bind selected intracellular targets inside living human cells and to be detected in vitreous sections. As a proof of concept, thioaminobenzoate‐, thionitrobenzoate‐coordinated gold nanoparticles are functionalized on their surface with SV40 Nuclear Localization Signal (NLS)‐containing peptides and 2 kDa polyethyleneglycols (PEG) by thiolate exchange to target the importin‐mediated nuclear machinery and facilitate cytosolic diffusion by shielding the AuNP surface from non‐specific binding to cell components, respectively. After delivery by electroporation into the cytoplasm of living human cells, the PEG‐coated AuNPs diffuse freely in the cytoplasm but do not enter the nucleus. Incorporation of NLS within the PEG coverage promotes a quick nuclear import of the nanoparticles in relation to the density of NLS onto the AuNPs. Cryo‐EM of vitreous cell sections demonstrate the presence of 2 nm AuNPs as single entities in the nucleus. Biofunctionalized AuNPs combined with live‐cell electroporation procedures are thus potent labeling tools for the identification of macromolecules in cellular cryo‐EM.