Mohammad Rafe Hatshan

The doping of engineered nanomaterials (ENMs) is a key tool for manipulating the properties of ENMs (e.g., electromagnetic, optical, etc.) for different therapeutic applications. However, adverse health outcomes and the cellular biointeraction of doped ENMs, compared to undoped counterparts, are not fully understood. Previously, we have shown that doping manganese oxide nanoparticles with ZnO (ZnO-MnO2 NPs) improved their catalytic properties. In this study, we assessed the toxicity of ZnO-MnO2 NPs in Raw 264.7 cells. NPs were prepared via an eco-friendly, co-precipitation method and characterized by several techniques, including transmission and scanning electron microscopy, X-ray diffraction, and Fourier transform infrared. The physicochemical properties of ZnO-MnO2 NPs, including size, morphology, and crystalline structure, were almost identical to MnO2 NPs. However, ZnO-MnO2 NPs showed slightly larger particle aggregates and negative charge in cell culture media. Exposure to ZnO-MnO2 NPs resulted in lower toxicity based on the cell viability and functional assay (phagocytosis) data. Exposure to both NPs resulted in the activation of the cell inflammatory response and the generation of reactive oxygen species (ROS). Despite this, exposure to ZnO-MnO2 NPs was associated with a lower toxicity profile, and it resulted in a higher ROS burst and the activation of the cell antioxidant system, hence indicating that MnO2 NP-induced toxicity is potentially mediated via other ROS-independent pathways. Furthermore, the cellular internalization of ZnO-MnO2 NPs was lower compared to MnO2 NPs, and this could explain the lower extent of toxicity of ZnO-MnO2 NPs and suggests Zn-driven ROS generation. Together, the findings of this report suggest that ZnO (1%) doping impacts cellular biointeraction and the consequent toxicological outcomes of MnO2 NPs in Raw 264.7 cells.

Eco-friendly preparation of metallic nanoparticles (NPs) is a greatly evolving field of scientific research. These types of NPs have gained substantial recognition from scientists, including chemists, chemical biologists and technologists, who have successfully exploited them for the fabrication of a variety of advanced nanodevices. Herein, silver (Ag) NPs were synthesized by a green approach using the aqueous extract of Vaccinium oxycoccos (cranberry), which not only reduced the silver ions but also stabilized the surface of the resultant Ag NPs. The formation of Ag NPs is confirmed by different analytical techniques, including powder X-ray diffraction, UV analysis, scanning electron microscopy (SEM), energy-dispersive X-ray (EDX), transmission electron microscopy (TEM) and Fourier-transform infrared spectroscopy (FT-IR). The UV analysis of the aqueous solution of the reaction mixture demonstrated an absorption band at ~450 nm, which is the typical peak of Ag NPs, leading to the confirmation of product formation. While the XRD confirmed the crystallinity of the sample and the formation of a face-centered cubic (fcc) structure, on the other hand, TEM revealed the presence of spherical NPs with an approximate size range between 5–30 nm. Furthermore, the as-obtained Ag NPs were subjected to thorough investigations to explore the biomedical potential of the sample. In this case, the Ag NPs demonstrated considerable antioxidant and antifungal properties towards various pathogens. In addition, Ag NPs also showed substantial inhibition of spore germination.