Petar Todorov

Some new hemorphin‐4 analogs with structures of Xxx‐Pro‐Trp‐Thr‐NH 2 and Tyr‐Yyy‐Trp‐Thr‐NH 2 , where Xxx is 2‐amino‐3‐(4−hydroxy‐2,6‐dimethylphenyl)propanoic acid or 2‐amino‐3‐(4‐dibenzylamino‐2,6‐dimethylphenyl)propanoic acid, and Yyy is (2 S ,4 S )‐4‐amino‐pyrrolidine‐2‐carboxylic acid, were synthesized and characterized by electrochemical and spectral analyses. In vivo anticonvulsant and antinociceptive activities of peptide derivatives were studied after intracerebroventricular injection in mice. The therapeutic effects of the modified peptides on seizures and pain in mice were evaluated to provide valuable insights into the potential applications of the novel compounds. Electrochemical characterization showed that the compounds behave as weak protolytes and that they are in a soluble, stable molecular form at physiological pH values. The antioxidant activity of the peptides was evaluated with voltammetric analyses, which were confirmed by applying the 2,2‐Diphenyl‐1‐picrylhydrazyl method. The compounds showed satisfactory results regarding their structural stability, reaching the desired centers for the manifestation of biological activity without hydrolysis processes at 37°C and physiological pH. Dm‐H4 and H4‐P1 exhibited 100% and 83% potency to suppress the psychomotor seizures in the 6‐Hz test compared to 67% activity of H4 . Notably, only the H4‐P1 had efficacy in blocking the tonic component in the maximal electroshock test with a potency comparable to H4 . All investigated peptides containing unnatural conformationally restricted amino acids showed antinociceptive effects. The analogs Db‐H4 and H4‐P1 showed the most pronounced and long‐lasting effect in both experimental models of pain induced by thermal and chemical stimuli. Dm‐H4 produced a dose‐dependent thermal antinociception and H4‐P2 inhibited only formalin‐induced pain behavior.