Sofia Guilherme

The harmful effects of pesticides can be extended beyond the exposure time scale. Appraisals combining exposure and long‐term post‐exposure periods appear as an unavoidable approach in pesticide risk assessment, thus allowing a better understanding of the real impact of agrochemicals in non‐target organisms. This study aimed to evaluate the progression of genetic damage in somatic and germ tissues of the crayfish Procambarus clarkii , also seeking for gender‐specificities, following exposure (7 days) to penoxsulam (23 μg L −1 ) and a post‐exposure (70 days) period. The same approach was applied to the model genotoxicant ethyl methanesulfonate (EMS; 5 mg L −1 ) as a complementary mean to improve knowledge on genotoxicity dynamics (induction vs. recovery). Penoxsulam induced DNA damage in all tested tissues, disclosing tissue‐ and gender‐specificities, where females showed to be more vulnerable than males in the gills, while males demonstrated higher susceptibility in what concerns internal organs, that is, hepatopancreas and gonad. Crayfish were unable to recover from the DNA damage induced by EMS in gills and hepatopancreas (both genders) as well as in spermatozoa. The genotoxicity in the hepatopancreas was only perceptible in the post‐exposure period. Oxidative DNA lesions were identified in hepatopancreas and spermatozoa of EMS‐exposed crayfish. The spermatozoa proved to be the most vulnerable cell type. It became clear that the characterization of the genotoxic hazard of a given agent must integrate a complete set of information, addressing different types of DNA damage, tissue‐ and gender‐specificities, as well as a long‐term appraisal of temporal progression of damage.