Valentyna Yuriivna Dielievska

There is a need for specific determination of blood group antigens on erythrocytes for improvement of the quality of hemotransfusion therapy. O, A, B, AB, and weak variants of A and B antigen (O washed erythrocytes with anti‐A or anti‐B antibody absorbing ability) were investigated for electrophoretic mobility with immunoglobulin G complement‐dependent antibodies. The contact of anti‐A heated serum and complement with A erythrocytes resulted in their decreased electrokinetic potential the contrary to the serum without anti‐A and anti‐B antibodies and increased after addition of the serum from O blood group person with anti‐A and anti‐B absorbing ability. Anti‐B heated serum with complement decreased electrokinetic potential of B erythrocytes. Whereas both anti‐A heated serum and serum from AB person with complement did not decrease the electrokinetic potential of O erythrocytes. Anti‐B heated serum, in the same manner, decreased the electrokinetic potential of O blood group erythrocytes with anti‐B absorbing ability. Anti‐А immunoglobulin G complement‐dependent antibodies decreased the electrokinetic potential of washed O erythrocytes with аnti‐A absorbing ability contrary to immunoglobulin M antibodies. Evaluation of the electrokinetic potential of erythrocytes with a set of immunoglobulin G complement‐dependent antibodies might be considered for blood group type detection.