Zhun Li

A facultative anaerobic, Gram-negative, non-motile, rod-shaped bacterial strain, designated N5 T , was obtained from the phycosphere microbiota of the marine planktonic dinoflagellate, Karlodinium veneficum . Strain N5 T showed growth on marine agar at 25 °C, pH 7 and 1 % (w/v) NaCl and produced a yellow colour. According to a phylogenetic study based on 16S rRNA gene sequences, strain N5 T has a lineage within the genus Gymnodinialimonas . The G+C content in the genome of strain N5 T is 62.9 mol% with a total length of 4 324 088 bp. The NCBI Prokaryotic Genome Annotation Pipeline revealed that the N5 T genome contained 4230 protein-coding genes and 48 RNA genes, including a 5S rRNA, 16S rRNA, 23S rRNA, 42 tRNA, and three ncRNAs. Genome-based calculations (genome-to-genome distance, average nucleotide identity and DNA G+C content) clearly indicated that the isolate represents a novel species within the genus Gymnodinialimonas . The predominant fatty acids were C 19 : 0 cyclo ω 8 c and feature 8 (comprising C 18 : 1 ω 6 c and/or C 18 : 1 ω 7 c ). The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine. The main respiratory quinone was Q-10. Based on its phenotypic, phylogenetic, genomic and chemotaxonomic features, strain N5 T represents a novel species of the genus Gymnodinialimonas , for which the name Gymnodinialimonas phycosphaerae sp. nov. is proposed. The type strain is N5 T (=KCTC 82362 T =NBRC 114899 T ).

A novel bacterial strain, designated as GY-18 T , was isolated from particulate material sampled from the Korean south coast. The bacterium was Gram-stain-negative, aerobic and rod-shaped. Oxidase and catalase were positive. Optimal growth was observed at 30 °C, pH 7.0, in the presence of 1 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene and whole-genome sequences suggested that strain GY-18 T was a member of the genus Dasania , but represented a separate lineage. The 16S rRNA gene sequence of strain GY-18 T showed high similarities to Dasania marina KCTC 12566 T (98.4 %), Halioglobus japonicus KCTC 23429 T (92.2 %), Marimicrobium arenosum KCTC 42300 T (92.4 %) and Seongchinamella unica KCTC 62383 T (92.2 %), respectively. The genome length of strain GY-18 T was 3 808 450 bp with a DNA G+C content of 47.8 mol %. The major cellular fatty acids of strain GY-18 T were summed feature 3 (comprising C 16 : 1 ω 7 c and/or C 16 : 1 ω 6 c ), C 16 : 0 and C 10 : 0 3-OH (>10 %). Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phospholipids were shown to be the major polar lipids. The predominant isoprenoid quinone was Q-8. Based on phylogenetic, phenotypic, chemotaxonomic and genomic features, we propose that strain GY-18 T represents a novel species in the genus Dasania of the family <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.160