Marie Curie ON-Tract PhD Position: Protein Engineering, Directed Evolution & Machine Learning for Biocatalysis University of Cambridge in United Kingdom

The University of Cambridge's Department of Biochemistry is offering a fixed-term Marie Curie ON-Tract PhD position focused on protein engineering of enzymes, combining in vitro directed evolution and machine learning-based approaches to advance biocatalysis for synthetic applications. This three-year doctoral opportunity is part of the Horizon Europe EU Marie Curie Network ON-TRACT, which brings together leading research groups across Europe and the UK to develop oligonucleotide-based therapeutics, including anti-cancer strategies.

The successful candidate will join Professor Florian Hollfelder's research group, engaging in cutting-edge research to develop ultrahigh throughput methods for the in vitro evolution of enzymes relevant to synthetic chemistry, particularly for oligonucleotide constructs such as chiral synthesis involving IREDs and ligases. The project will utilize diverse enzyme libraries generated by error-prone PCR, site-saturation mutagenesis, and transposon-based InDel mutagenesis. In vitro selections will be followed by advanced techniques such as long-read nanopore sequencing and deep mutational scanning to analyze screening outputs.

Data generated from these experiments will be interpreted using regression and tree-based machine learning algorithms, enabling the identification of improved enzyme mutants and the development of mechanistic hypotheses. The ON-TRACT network offers extensive collaborative opportunities, providing training in organic synthesis, cell biology, medical research, and more, ensuring a broad skillset for the successful applicant.

Applicants should hold a first or upper second class degree in chemistry, biochemistry, or a closely related discipline. Experience with molecular and chemical biology techniques is essential, and additional skills in assay development, enzyme kinetics, DNA library generation, structural biology, organic synthesis, high-throughput screening, mechanistic enzymology, bioinformatics, and microfluidic engineering are highly desirable. Candidates are expected to submit a cover letter detailing their research interests, a comprehensive CV with exam marks and practical experience, a list of publications, and contact information for at least two referees familiar with their research work.

The position is funded for three years, from 1st February 2026 to 31st January 2029. While specific funding details are not provided, the role is part of a prestigious Marie Curie network, which typically offers competitive support. The University of Cambridge is committed to equality, diversity, and inclusion, and encourages applications from all backgrounds. Applicants must be eligible to live and work in the UK.

To apply, candidates should use the online application link provided. For informal scientific enquiries, contact Professor Florian Hollfelder at [email protected]. For questions regarding the application process, reach out to the Biochemistry HR Team at [email protected]. Please reference PH48293 in all correspondence related to this vacancy.