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Thomas Frischmuth

1 year ago

PhD Position: Development of Click Chemistry-Enabled NGS Library Technology for Direct RNA Profiling University of Udine in Italy

Degree Level

PhD

Field of study

Biochemistry

Funding

Funded PhD Project (Students Worldwide)

Deadline

Expired

Country flag

Country

Italy

University

University of Udine

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Keywords

Biochemistry
Chemistry
Biotechnology
Organic Chemistry
Biology
Rna Biology
Nanopore Sequencing
Rna-seq
Ngs
Nucleic Acid Chemistry
Click Chemistry
Non-coding Rna
Bioanalytical Chemistry

About this position

This PhD position focuses on the development of next-generation sequencing (NGS) library technology for direct RNA profiling using click chemistry, specifically targeting non-coding RNA (ncRNA). The project aims to exploit click-chemistry-mediated ligation to generate libraries for direct RNA sequencing without the need for reverse transcription or amplification, utilizing the Oxford Nanopore Technology (ONT) platform. Key objectives include optimizing the incorporation of azido- and alkyne-modified nucleotides at both ends of RNA, establishing optimal conditions for copper-catalyzed azide-alkyne cycloaddition (CuAAC) reactions between RNA and ONT sequencing adaptors, and screening click-modified adaptors for best performance in both sequencing directions. The overarching goal is to streamline RNA library generation for direct sequencing on the ONT platform, reducing input RNA requirements and potentially increasing coverage of the non-coding transcriptome, including direct epitranscriptome analysis.

The host institution, baseclick GmbH, is a specialized company near Munich, Germany, providing fully equipped laboratories for chemical and biochemical research, including Oxford Nanopore sequencing capabilities. The project is part of the INT2ACT Doctoral Network and involves strong collaboration with research groups in Italy and Poland, with expected secondments and travel. Applicants must have a Master's degree in a relevant field, research experience in biochemical techniques, and proficiency in English.

Funding is provided according to EU MSCA regulations, and the application deadline is January 31, 2026. For more information and to apply, candidates should visit the INT2ACT recruitment web page and prepare the required documents as specified.

Funding details

Funded PhD Project (Students Worldwide)

What's required

Applicants must hold a Master's degree in Biotechnology, Biochemistry, Organic Chemistry, or related fields. They must not already possess a doctoral degree at the date of recruitment. Candidates must comply with the mobility rule, meaning they must not have resided or carried out their main activity in Germany for more than 12 months in the 36 months immediately before recruitment. Research experience in biochemical techniques is required, with preference for experience in nucleic acids and chemical reactions. Proficiency in English is required, demonstrated by an English test (IELTS, TOEFL, Cambridge English) unless exempted by nationality or prior English-taught degree. Good oral and written communication skills are essential.

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Professors

Dr. at INT2ACT

University Name
Country flag

Italy

Research Interests

Organic Chemistry

Biochemistry

Biology

Biotechnology

Biomedical Engineering

Dr. at INT2ACT

University Name
Country flag

Italy

Research Interests

Organic Chemistry

Biochemistry

Nucleic Acid Chemistry

Nanopore Sequencing

Ngs