Gerard Roelfes

University of Groningen

Netherlands

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Articles (22)

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2025

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2021

Collaborators (10)

Ruben Marcel de Boer

Technical University of Denmark

DENMARK

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Andy-Mark Thunnissen

Assistant Professor

University of Groningen

NETHERLANDS

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Bart Brouwer

Vrije Universiteit Amsterdam

NETHERLANDS

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Hugo van Ingen

NETHERLANDS

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Lars Longwitz

University of Groningen

NETHERLANDS

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Reuben Leveson-Gower

Assistant Professor

TU Delft

NETHERLANDS

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Rifka Vlijm

Associate professor

University of Groningen

NETHERLANDS

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Ruben Maaskant

University of Groningen

NETHERLANDS

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Oscar P. Kuipers

University of Groningen

NETHERLANDS

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Marc Baldus

Utrecht University

NETHERLANDS

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Gerard Roelfes

Research Interests

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Articles (22)

Activity-enhancing mutations in an LmrR-based artificial metalloenzyme destabilize the protein scaffold and alter its conformational plasticity.

An artificial copper-Michaelase featuring a genetically encoded bipyridine ligand for asymmetric additions to nitroalkenes

Artificial Gold Enzymes Using a Genetically Encoded Thiophenol‐Based Noble‐Metal‐Binding Ligand

Evolutionary Specialization of a Promiscuous Designer Enzyme

Genetically encoded 3-aminotyrosine as catalytic residue in a designer Friedel-Crafts alkylase

A copper enzyme for Lewis acid biocatalysis

Computation-guided engineering of distal mutations in an artificial enzyme

Boron catalysis in a designer enzyme

Selective Aza-Michael Addition to Dehydrated Amino Acids in Natural Antimicrobial Peptides

Boron Designer Enzyme with a Hybrid Catalytic Dyad

Noncanonical Amino Acids: Bringing New-to-Nature Functionalities to Biocatalysis

Artificial metalloenzymes

In Vivo Biocatalytic Cascades Featuring an Artificial-Enzyme-Catalysed New-to-Nature Reaction**

Biocatalytic Friedel-Crafts Reactions

Tandem Friedel-Crafts-Alkylation-Enantioselective-Protonation by Artificial Enzyme Iminium Catalysis

In Vivo Assembly of Artificial Metalloenzymes and Application in Whole‐Cell Biocatalysis**

Repurposed and artificial heme enzymes for cyclopropanation reactions

Synergistic Catalysis of Tandem Michael Addition/Enantioselective Protonation Reactions by an Artificial Enzyme

Unlocking Iminium Catalysis in Artificial Enzymes to Create a Friedel-Crafts Alkylase

Chemogenetic Tags with Probe Exchange for Live-Cell Fluorescence Microscopy

Merging Whole-cell Biosynthesis of Styrene and Transition-metal Catalyzed Derivatization Reactions

Rapid and selective chemical editing of Ribosomally synthesized and Post-translationally modified Peptides (RiPPs) via Cu(II)-catalyzed β-borylation of dehydroamino acids

Collaborators (10)

Ruben Marcel de Boer

Andy-Mark Thunnissen

Bart Brouwer

Hugo van Ingen

Lars Longwitz

Reuben Leveson-Gower

Rifka Vlijm

Ruben Maaskant

Oscar P. Kuipers

Marc Baldus

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