Paul Ross
Professor at University College Cork
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Articles (11)
Winogradskyella bathintestinalis sp. nov., isolated from the intestine of the deep-sea loosejaw dragonfish, Malacosteus niger
A novel bacterial strain, APC 3343 T , was isolated from the intestine of a deep-sea loosejaw dragon fish, Malacosteus niger , caught at a depth of 1000 m in the Northwest Atlantic Ocean. Cells were aerobic, rod-shaped, yellow/orange-pigmented, non-motile and Gram-negative. Growth of strain APC 3343 T was observed at 4–30 °C (optimum, 21–25 °C), pH 5.5–10 (optimum, pH 7–8) and 0.5–8 % (w/v) NaCl (optimum, 2–4 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain APC 3343 T was most closely related to members of the genus Winogradskyella , with the most closely related type strains being Winogradskyella algae Kr9-9 T (98.46 % identity), Winogradskyella damuponensis F081-2 T (98.07 %), Winogradskyella eximia CECT 7946 T (97.93 %), Winogradskyella litoriviva KMM 6491 T (97.79 %) and Winogradskyella endarachnes HL2-2 T (97.79 %). Major fatty acids (>10 % of total) were iso-C 16 : 0 3-OH, iso-C 15 : 0 , anteiso-C 15 : 0 and iso-C 17 : 0 3-OH. The predominant respiratory quinone was menaquinone-6 (MK-6). Polar lipids were phosphatidylethanolamine, three unknown aminolipids and eight unknown lipids. The draft genome sequence was 3.8 Mb in length with a G+C content of 33.43 mol%. Based on the phenotypic characteristics and phylogenetic analysis, strain APC 3343 T is deemed to be a novel species of the genus Winogradskyella <
Year:
2023
After a century of nisin research - where are we now?
It is almost a century since nisin was discovered in fermented milk cultures, coincidentally in the same year that penicillin was first described. Over the last 100 years this small, highly modified pentacyclic peptide has not only found success in the food industry as a preservative but has also served as the paradigm for our understanding of the genetic organization, expression, and regulation of genes involved in lantibiotic biosynthesis—one of the few cases of extensive post-translation modification in prokaryotes. Recent developments in understanding the complex biosynthesis of nisin have shed light on the cellular location of the modification and transport machinery and the co-ordinated series of spatio-temporal events required to produce active nisin and provide resistance and immunity. The continued unearthing of new natural variants from within human and animal gastrointestinal tracts has sparked interest in the potential application of nisin to influence the microbiome, given the growing recognition of the role the gastrointestinal microbiota plays in health and disease. Moreover, interdisciplinary approaches have taken advantage of biotechnological advancements to bioengineer nisin to produce novel variants and expand nisin functionality for applications in the biomedical field. This review will discuss the latest progress in these aspects of nisin research.
Year:
2023
Collaborators (5)
Crystal Johnson, PhD
Assistant Professor
University of Oklahoma Health Sciences Center
Ghjuvan Grimaud
Assistant Professor
Lund University
Colin Hill
Lecturer/Professor
University College Cork
Song Miao
Adjunct Professor
University College Cork
Colin Hill
University College Cork

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