Vasanthi Jayaraman

Kainate receptors are a subtype of ionotropic glutamate receptors that form transmembrane channels upon binding glutamate. Here, we have investigated the mechanism of partial agonism in heteromeric GluK2/K5 receptors, where the GluK2 and GluK5 subunits have distinct agonist binding profiles. Using single‐molecule Förster resonance energy transfer, we found that at the bi‐lobed agonist‐binding domain, the partial agonist AMPA‐bound receptor occupied intermediate cleft closure conformational states at the GluK2 cleft, compared to the more open cleft conformations in apo form and more closed cleft conformations in the full agonist glutamate‐bound form. In contrast, there is no significant difference in cleft closure states at the GluK5 agonist‐binding domain between the partial agonist AMPA‐ and full agonist glutamate‐bound states. Additionally, unlike the glutamate‐bound state, the dimer interface at the agonist‐binding domain is not decoupled in the AMPA‐bound state. Our findings suggest that partial agonism observed with AMPA binding is mediated primarily due to differences in the GluK2 subunit, highlighting the distinct contributions of the subunits towards activation.