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U.S. Environmental Protection Agency
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EPA Development and Qualification of In Vitro Blood-Brain Barrier Models for Environmental Chemical Screening U.S. Environmental Protection Agency (EPA) in United States
Degree Level
Master's, Postdoc
Field of study
Chemistry
Funding
Stipend of $52,693.00–$64,453.00 per year.
Deadline
Dec 18, 2026
Country
United States
University
U.S. Environmental Protection Agency
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About this position
This research opportunity at the U.S. Environmental Protection Agency focuses on the development and qualification of in vitro blood-brain barrier (BBB) models for environmental chemical screening. The work is based in the EPA’s Office of Chemical Safety and Pollution Prevention in Durham, North Carolina, within the Risk Assessment Support Division, where human-relevant methods are used to improve assessment of chemical hazards and risks.
The participant will join a team developing New Approach Methodologies that support agency efforts to generate human-relevant information while reducing reliance on vertebrate animal testing. The project centers on establishing and optimizing BBB model systems, running chemical exposure, permeability, and transport studies, and using the resulting data to support in vitro-to-in vivo extrapolation and physiologically based pharmacokinetic modeling of chemical disposition and brain exposure.
The research environment includes physiologically relevant in vitro models, high-throughput screening approaches, and computational and informatics tools used to advance predictive toxicological assessment. The participant will also gain exposure to human primary cell cultures, high-content imaging and image analysis, toxicokinetic endpoint assessment, gene expression assays, and advanced culture systems such as 3D spheroids, organoids, and tissue chips.
Beyond experimental work, the opportunity includes scientific literature curation, preparation of reports, presentations at national meetings, and manuscript writing for peer-reviewed publication. The named mentor for the project is Sreenivasa Ramaiahgari at EPA.
Funding details
Stipend of $52,693.00–$64,453.00 per year.
What's required
The qualified candidate should have received or be currently pursuing a bachelor's or master's degree in biology, cell/molecular biology, biochemistry, toxicology, environmental science, or a closely related field. U.S. citizenship is required. Preferred qualifications include experience with in vitro cell culture and assay development, familiarity with high-content imaging systems (epifluorescence or confocal microscopy), experience with immunofluorescence and immunohistochemistry, proficiency in molecular biology techniques including DNA/RNA extractions, knowledge of basic statistical methods, skill in communicating scientific research through posters and oral presentations, and experience with or courses in open-source programming languages such as R or Python. The candidate should be highly self-motivated, well-organized, innovative, and able to work independently and collaboratively. A successful background investigation is required for on-boarding at EPA, and documents must be in English or include an official English translation.
How to apply
Scroll to the bottom of the opportunity and click APPLY. Submit an application, transcripts, a current resume/CV, and two educational or professional recommendations. Make sure all documents are in English or have official English translations.
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