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Cees Dekker

Professor at Delft University of Technology

Delft University of Technology
Country flagNetherlands

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Positions (1)

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Cees Dekker

University Name
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Delft University of Technology

The Cees Dekker Lab at Delft University of Technology in the Netherlands is recruiting both PhD students and postdoctoral researchers for several cutting-edge projects in biophysics, nanoscience, and synthetic cell research. Current openings include positions focused on in vitro reconstitution of archaeal cell division systems, peptide sequencing using DNA origami nanopores, development of microfluidics circuitry for synthetic cells, and studies of mimics of nuclear pore complexes and peroxisomes. The lab is internationally recognized and seeks outstanding experimentalists with backgrounds in biophysics, single-molecule techniques, synthetic cells, optical imaging, nanosciences, or molecular/cellular biology. Diversity is actively fostered, and female candidates are especially encouraged to apply. Postdoctoral positions are offered for 2-4 years, and PhD positions for 4 years. Interested applicants should send a cover letter, CV with publication list, and contact information for at least three references to Professor Cees Dekker. Application materials may be reviewed by CDlab group members. For more details, visit the lab website.

Articles (11)

Year:

2023

In most bacteria, chromosome segregation is driven by the ParABS system where the CTPase protein ParB loads at the parS site to trigger the formation of a large partition complex. Here, we present in vitro studies of the partition complex for Bacillus subtilis ParB, using single-molecule fluorescence microscopy and AFM imaging to show that transient ParB–ParB bridges are essential for forming DNA condensates. Molecular Dynamics simulations confirm that condensation occurs abruptly at a critical concentration of ParB and show that multimerization is a prerequisite for forming the partition complex. Magnetic tweezer force spectroscopy on mutant ParB proteins demonstrates that CTP hydrolysis at the N-terminal domain is essential for DNA condensation. Finally, we show that transcribing RNA polymerases can steadily traverse the ParB–DNA partition complex. These findings uncover how ParB forms a stable yet dynamic partition complex for chromosome segregation that induces DNA condensation and segregation while enabling replication and transcription.

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2023

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2023

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2023

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2022

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2022

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2022

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2022

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2022

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2022

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2022

Collaborators (13)

Yujia Qing

-

UNITED KINGDOM
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Xin Shi

Assistant Professor

KU Leuven

BELGIUM
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Sonja Schmid

Wageningen University & Research

NETHERLANDS
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Weria Pezeshkian

Københavns Universitet

DENMARK
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Roman Barth

Delft University of Technology

NETHERLANDS
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Farbod Alijani

Associate Professor

Delft University of Technology

NETHERLANDS
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Jacob Willem Jozef Kerssemakers

Delft University of Technology

NETHERLANDS
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Chenyu Wen

Assistant Professor

Uppsala University

SWEDEN
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Winfried Weissenhorn

Université Grenoble Alpes

FRANCE
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Aleksandre Japaridze

Delft University of Technology

NETHERLANDS
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Peter G. Steeneken

-

NETHERLANDS
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Erwin Frey

Professor

Ludwig-Maximilians-Universität München

GERMANY
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Stephan Gruber

Associate Professor

University of Lausanne

SWITZERLAND
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