Cees Dekker
Professor at Delft University of Technology
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Positions (1)
Cees Dekker
Delft University of Technology
The Cees Dekker Lab at Delft University of Technology in the Netherlands is recruiting both PhD students and postdoctoral researchers for several cutting-edge projects in biophysics, nanoscience, and synthetic cell research. Current openings include positions focused on in vitro reconstitution of archaeal cell division systems, peptide sequencing using DNA origami nanopores, development of microfluidics circuitry for synthetic cells, and studies of mimics of nuclear pore complexes and peroxisomes. The lab is internationally recognized and seeks outstanding experimentalists with backgrounds in biophysics, single-molecule techniques, synthetic cells, optical imaging, nanosciences, or molecular/cellular biology. Diversity is actively fostered, and female candidates are especially encouraged to apply. Postdoctoral positions are offered for 2-4 years, and PhD positions for 4 years. Interested applicants should send a cover letter, CV with publication list, and contact information for at least three references to Professor Cees Dekker. Application materials may be reviewed by CDlab group members. For more details, visit the lab website.
Articles (11)
Year:
2023
In most bacteria, chromosome segregation is driven by the ParABS system where the CTPase protein ParB loads at the parS site to trigger the formation of a large partition complex. Here, we present in vitro studies of the partition complex for Bacillus subtilis ParB, using single-molecule fluorescence microscopy and AFM imaging to show that transient ParB–ParB bridges are essential for forming DNA condensates. Molecular Dynamics simulations confirm that condensation occurs abruptly at a critical concentration of ParB and show that multimerization is a prerequisite for forming the partition complex. Magnetic tweezer force spectroscopy on mutant ParB proteins demonstrates that CTP hydrolysis at the N-terminal domain is essential for DNA condensation. Finally, we show that transcribing RNA polymerases can steadily traverse the ParB–DNA partition complex. These findings uncover how ParB forms a stable yet dynamic partition complex for chromosome segregation that induces DNA condensation and segregation while enabling replication and transcription.
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2023
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2022
Collaborators (13)
Yujia Qing
-
Xin Shi
Assistant Professor
KU Leuven
Sonja Schmid
Wageningen University & Research
Weria Pezeshkian
Københavns Universitet
Roman Barth
Delft University of Technology
Farbod Alijani
Associate Professor
Delft University of Technology
Jacob Willem Jozef Kerssemakers
Delft University of Technology
Chenyu Wen
Assistant Professor
Uppsala University
Winfried Weissenhorn
Université Grenoble Alpes
Aleksandre Japaridze
Delft University of Technology
Peter G. Steeneken
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Erwin Frey
Professor
Ludwig-Maximilians-Universität München
Stephan Gruber
Associate Professor
University of Lausanne
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