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Hiroshi Ueno

University of Tokyo

Japan

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Research Interests

Biochemistry

90%

Biochemical Engineering

80%

Allosteric Regulation

60%

Membrane Transport

50%

Electron Transfer

40%

Biocatalysis

40%

Enzymology

30%

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Articles19

Engineering of ATP synthase for enhancement of proton-to-ATP ratio

Article Link

Year:

2025

Functional and Structural Characterization of F1-ATPase with common ancestral core domains in stator ring

Article Link

Year:

2025

Engineering of IF1‐susceptive bacterial F1‐ATPase

Article Link

Year:

2024

The series of conformational states adopted by rotorless F1-ATPase during its hydrolysis cycle

Article Link

Year:

2024

Genetic Perturbation Alters Functional Substates in Alkaline Phosphatase

Article Link

Year:

2023

Six states of Enterococcus hirae V-type ATPase reveals non-uniform rotor rotation during turnover

Article Link

Year:

2023

Digital Cascade Assays for ADP- or ATP-Producing Enzymes Using a Femtoliter Reactor Array Device

Article Link

Year:

2023

Molecular mechanism on forcible ejection of ATPase inhibitory factor 1 from mitochondrial ATP synthase

Article Link

Year:

2023

Direct identification of the rotary angle of ATP cleavage in F1-ATPase from Bacillus PS3

Article Link

Year:

2023

Rotary properties of hybrid F1-ATPases consisting of subunits from different species

Article Link

Year:

2023

Direct observation of stepping rotation of V-ATPase reveals rigid component in coupling between V o and V 1 motors

Article Link

Year:

2022

The six steps of the complete F1-ATPase rotary catalytic cycle

Article Link

Year:

2021

Amplification of over 100 kbp DNA from Single Template Molecules in Femtoliter Droplets

Article Link

Year:

2021

Elucidation and control of low and high active populations of alkaline phosphatase molecules for quantitative digital bioassay

Article Link

Year:

2021

Tracking the 3D Rotational Dynamics in Nanoscopic Biological Systems

Article Link

Year:

2020

Rotary catalysis of bovine mitochondrial F 1 -ATPase studied by single-molecule experiments

Article Link

Year:

2020

The 3 × 120° rotary mechanism of Paracoccus denitrificans F 1 -ATPase is different from that of the bacterial and mitochondrial F 1 -ATPases

Article Link

Year:

2020

Monodisperse Liposomes with Femtoliter Volume Enable Quantitative Digital Bioassays of Membrane Transporters and Cell-Free Gene Expression

Article Link

Year:

2020

Tight Chemomechanical Coupling of the F1 Motor Relies on Structural Stability

Article Link

Year:

2020

Collaborators12

Duncan G. G. McMillan

Delft University of Technology

NETHERLANDS

RYOTA IINO

教授/Professor

分子科学研究所/Institute for Molecular Science

JAPAN

Hidehito Tochio

教授 / Professor

Kyoto University

JAPAN

Shingo Sotoma

Assistant professor

Osaka University

JAPAN

Kazuhito Tabata

Associate prof.

The Univeristy of Tokyo

JAPAN

Akihiro Otomo

助教/Assistant Professor

分子科学研究所/Institute for Molecular Science

JAPAN

Hiroyuki Noji

JAPAN

Nobuhiko Tokuriki

Professor

The University of British Columbia

CANADA

Alastair Stewart

Lab Head

Victor Chang Cardiac Research Institute

AUSTRALIA

Takeshi Murata

Chiba University

JAPAN

Chun-Biu Li

Associate Professor

Stockholm University

SWEDEN

Kei-ichi Okazaki

准教授/Associate Professor

JAPAN

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Hiroshi Ueno

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Articles19

Engineering of ATP synthase for enhancement of proton-to-ATP ratio

Functional and Structural Characterization of F<sub>1</sub>-ATPase with common ancestral core domains in stator ring

Engineering of IF<sub>1</sub>‐susceptive bacterial F<sub>1</sub>‐ATPase

The series of conformational states adopted by rotorless F1-ATPase during its hydrolysis cycle

Genetic Perturbation Alters Functional Substates in Alkaline Phosphatase

Six states of Enterococcus hirae V-type ATPase reveals non-uniform rotor rotation during turnover

Digital Cascade Assays for ADP- or ATP-Producing Enzymes Using a Femtoliter Reactor Array Device

Molecular mechanism on forcible ejection of ATPase inhibitory factor 1 from mitochondrial ATP synthase

Direct identification of the rotary angle of ATP cleavage in F<sub>1</sub>-ATPase from Bacillus PS3

Rotary properties of hybrid F<sub>1</sub>-ATPases consisting of subunits from different species

Direct observation of stepping rotation of V-ATPase reveals rigid component in coupling between V o and V 1 motors

The six steps of the complete F1-ATPase rotary catalytic cycle

Amplification of over 100 kbp DNA from Single Template Molecules in Femtoliter Droplets

Elucidation and control of low and high active populations of alkaline phosphatase molecules for quantitative digital bioassay

Tracking the 3D Rotational Dynamics in Nanoscopic Biological Systems

Rotary catalysis of bovine mitochondrial F 1 -ATPase studied by single-molecule experiments

The 3 × 120° rotary mechanism of Paracoccus denitrificans F 1 -ATPase is different from that of the bacterial and mitochondrial F 1 -ATPases

Monodisperse Liposomes with Femtoliter Volume Enable Quantitative Digital Bioassays of Membrane Transporters and Cell-Free Gene Expression

Tight Chemomechanical Coupling of the F1 Motor Relies on Structural Stability

Collaborators12

Duncan G. G. McMillan

RYOTA IINO

Hidehito Tochio

Shingo Sotoma

Kazuhito Tabata

Akihiro Otomo

Hiroyuki Noji

Nobuhiko Tokuriki

Alastair Stewart

Takeshi Murata

Chun-Biu Li

Kei-ichi Okazaki

How do I reach out?

Hiroshi Ueno

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Research Interests

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Articles19

Engineering of ATP synthase for enhancement of proton-to-ATP ratio

Functional and Structural Characterization of F&lt;sub&gt;1&lt;/sub&gt;-ATPase with common ancestral core domains in stator ring

Engineering of IF<sub>1</sub>‐susceptive bacterial F<sub>1</sub>‐ATPase

The series of conformational states adopted by rotorless F1-ATPase during its hydrolysis cycle

Genetic Perturbation Alters Functional Substates in Alkaline Phosphatase

Six states of Enterococcus hirae V-type ATPase reveals non-uniform rotor rotation during turnover

Digital Cascade Assays for ADP- or ATP-Producing Enzymes Using a Femtoliter Reactor Array Device

Molecular mechanism on forcible ejection of ATPase inhibitory factor 1 from mitochondrial ATP synthase

Direct identification of the rotary angle of ATP cleavage in F&lt;sub&gt;1&lt;/sub&gt;-ATPase from Bacillus PS3

Rotary properties of hybrid F&lt;sub&gt;1&lt;/sub&gt;-ATPases consisting of subunits from different species

Direct observation of stepping rotation of V-ATPase reveals rigid component in coupling between V o and V 1 motors

The six steps of the complete F1-ATPase rotary catalytic cycle

Amplification of over 100 kbp DNA from Single Template Molecules in Femtoliter Droplets

Elucidation and control of low and high active populations of alkaline phosphatase molecules for quantitative digital bioassay

Tracking the 3D Rotational Dynamics in Nanoscopic Biological Systems

Rotary catalysis of bovine mitochondrial F 1 -ATPase studied by single-molecule experiments

The 3 × 120° rotary mechanism of Paracoccus denitrificans F 1 -ATPase is different from that of the bacterial and mitochondrial F 1 -ATPases

Monodisperse Liposomes with Femtoliter Volume Enable Quantitative Digital Bioassays of Membrane Transporters and Cell-Free Gene Expression

Tight Chemomechanical Coupling of the F1 Motor Relies on Structural Stability

Collaborators12

Duncan G. G. McMillan

RYOTA IINO

Hidehito Tochio

Shingo Sotoma

Kazuhito Tabata

Akihiro Otomo

Hiroyuki Noji

Nobuhiko Tokuriki

Alastair Stewart

Takeshi Murata

Chun-Biu Li

Kei-ichi Okazaki

How do I reach out?

Functional and Structural Characterization of F<sub>1</sub>-ATPase with common ancestral core domains in stator ring

Direct identification of the rotary angle of ATP cleavage in F<sub>1</sub>-ATPase from Bacillus PS3

Rotary properties of hybrid F<sub>1</sub>-ATPases consisting of subunits from different species