Irina Nazarenko

Head of the Exosome and Tumor Biology group

University Medical Center Freiburg

Germany

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About

Irina Nazarenko is the Head of the Exosome and Tumor Biology group at Medical Centre University Freiburg, Germany. Her research focuses on extracellular vesicles and their role in tumor biology, as evident from her recent publications on topics such as tumorigenicity, proteomic profiling, and the implications of small RNA cargo in aging. She also investigates the interactions of extracellular vesicles with various cellular processes, particularly in the context of cancer and infectious diseases.

Articles (10)

Limbal epithelial progenitor cells (LEPC) rely on their niche environment for proper functionality and self-renewal. While extracellular vesicles (EV), specifically small EVs (sEV), have been proposed to support LEPC homeostasis, data on sEV derived from limbal niche cells like limbal mesenchymal stromal cells (LMSC) remain limited, and there are no studies on sEVs from limbal melanocytes (LM). In this study, we isolated sEV from conditioned media of LMSC and LM using a combination of tangential flow filtration and size exclusion chromatography and characterized them by nanoparticle tracking analysis, transmission electron microscopy, Western blot, multiplex bead arrays, and quantitative mass spectrometry. The internalization of sEV by LEPC was studied using flow cytometry and confocal microscopy. The isolated sEVs exhibited typical EV characteristics, including cell-specific markers such as CD90 for LMSC-sEV and Melan-A for LM-sEV. Bioinformatics analysis of the proteomic data suggested a significant role of sEVs in extracellular matrix deposition, with LMSC-derived sEV containing proteins involved in collagen remodeling and cell matrix adhesion, whereas LM-sEV proteins were implicated in other cellular bioprocesses such as cellular pigmentation and development. Moreover, fluorescently labeled LMSC-sEV and LM-sEV were taken up by LEPC and localized to their perinuclear compartment. These findings provide valuable insights into the complex role of sEV from niche cells in regulating the human limbal stem cell niche.

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2024

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2024

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2024

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2024

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2023

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2023

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2023

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2023

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2022

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2022

Collaborators (86)

VIKRAMSINGH GUJAR

Assistant Professor

University of Oklahoma Health Sciences Center

UNITED STATES

Irina Nazarenko

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About

Articles (10)

Enrichment, Characterization, and Proteomic Profiling of Small Extracellular Vesicles Derived from Human Limbal Mesenchymal Stromal Cells and Melanocytes

Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches.

Corrigendum to "Tumorigenicity of IL-1α- and IL-1β-Deficient Fibrosarcoma Cells" [Neoplasia, Volume 10, Issue 6, June 2008, Pages 549-562].

Separation of small extracellular vesicles (sEV) from human blood by Superose 6 size exclusion chromatography.

Tspan6 stimulates the chemoattractive potential of breast cancer cells for B cells in an EV- and LXR-dependent manner

SARS-CoV-2 infection of airway organoids reveals conserved use of Tetraspanin-8 by Ancestral, Delta, and Omicron variants

Ageing-associated small RNA cargo of extracellular vesicles

MIBlood-EV: Minimal information to enhance the quality and reproducibility of blood extracellular vesicle research.

Airway basal cells show a dedifferentiated KRT17highPhenotype and promote fibrosis in idiopathic pulmonary fibrosis

Highly Sensitive Nanomagnetic Quantification of Extracellular Vesicles by Immunochromatographic Strips: A Tool for Liquid Biopsy

Collaborators (86)

VIKRAMSINGH GUJAR

Ramaroson Andriantsitohaina

Oscar Wiklander

Pia Siljander

Elena Martens-Uzunova

Michele Zanoni

Eduardo Reátegui

Sveva Bollini

Kirsty Danielson

Miguel Flores-Bellver

Anna Krasnodembskaya

Lucia Paolini

Jameel Inal

Julie Saugstad

Nicole Ludwig

Marta Garcia-Contreras

Fatemeh Momen-Heravi

Albano Caceres-Verschae

Fabian Michael Kern

Jennifer Jones

Maiken Mellergaard

Kimberley Louise Alexander

Christian Neri

Ann M Wehman

Lesley Cheng

Naresh Polisetti

Rachel Crossland

Daniel Kim

Suman Dutta

Deborah Goberdhan

FRANCESC E. BORRAS

Jason Webber

Mona Batish

Aurélie Ledreux

Tsuneya Ikezu

Cécile MALNOU

Mikael Klingeborn

John Miklavcic

Irundika Dias

Ayesha Saleem

Ole Østergaard

Michael P Rimmer

Mathilde Mosser

Sigrun Lange

Jeroen Roose

Manuel Varas-Godoy

Kenny Man

Somchai Chutipongtanate

Benedetta Bussolati

Janis A Müller

Richard Chahwan

Martin Simon

Marzena Kurzawa-Akanbi

Annalisa RADEGHIERI

Martin van Royen

Naveed Akbar

Riccardo Natoli

Christian P. Pallasch

Elisa Tonoli

Irma Schabussova

Meike Saul

Orazio Fortunato

Juhee Park