Alice Vrielink
AustraliaResearch Interests
Explore related searches
Contact this professor
Get a free first email built on one of their papers, ready for you to edit and send yourself.
Articles (13)
Burkholderia pseudomallei is a saprophytic Gram‐negative bacillus that can cause the disease melioidosis. Although B. pseudomallei is a recognised member of terrestrial soil microbiomes, little is known about its contribution to the saprophytic degradation of polysaccharides within its niche. For example, while chitin is predicted to be abundant within terrestrial soils the chitinolytic capacity of B. pseudomallei is yet to be defined. This study identifies and characterises a putative glycoside hydrolase, bpsl0500 , which is expressed by B. pseudomallei K96243. Recombinant BPSL0500 was found to exhibit activity against substrate analogues and GlcNAc disaccharides relevant to chitinolytic N ‐acetyl‐β‐ d ‐hexosaminidases. In B. pseudomallei, bpsl0500 was found to be essential for both N ‐acetyl‐β‐ d ‐hexosaminidase activity and chitooligosaccharide metabolism. Furthermore, bpsl0500 was also observed to significantly affect biofilm deposition. These observations led to the identification of BPSL0500 activity against model disaccharide linkages that are present in biofilm exopolysaccharides, a feature that has not yet been described for chitinolytic enzymes. The results in this study indicate that chitinolytic N ‐acetyl‐β‐ d ‐hexosaminidases like bpsl0500 may facilitate biofilm disruption as well as chitin assimilation, providing dual functionality for saprophytic bacteria such as B. pseudomallei within the competitive soil microbiome.
Year:
2024
Year:
2024
Year:
2024
Year:
2023
Year:
2023
Year:
2022
Year:
2022
Year:
2022
Year:
2021
Year:
2021
Year:
2021
Year:
2020
Year:
2020
Collaborators (66)
Dustin Duncan
Assistant Professor
Brock University
Charles Bond
University of Western Australia
Jadel M. Kratz
Head of Discovery and Partnerships LATAM
-
Iain Greig
University of Aberdeen
Danika Hope
University in Western Australia
Davide Maria Ferraris
Associate Professor
Università degli Studi del Piemonte Orientale
Kavita Gadar
University of Birmingham
Nathan Merrill
University of Michigan
Teemu Haikarainen
Tampere University
Peter Arthur
University of Western Australia
Giulia Girolimetti
Università del Salento
Heng Qiu
University of Western Australia
Luke Smithers
University of Western Australia
Jiyoun Lee
Professor
Sungshin Women's University
Timothy O'Meara
Harvard University
Masfique Mehedi
Assistant Professor
North Dakota State University
Keith Stubbs
University of Western Australia
Catherine A. Rinaldi
University of Western Australia
Kai Chen
University of Western Australia
GOKHAN CILDIR
University of South Australia
Andrew Marshall
University of Western Australia
Brandon Pressly
Denali Therapeutics
Rachael Zemek
University of Western Australia
Gracjan Michlewski
Professor
International Institute of Molecular and Cell Biology
Ashleigh Paparella
Lecturer
University of Sydney
Clare Moran
University of Melbourne
Jennifer Tickner
University of Western Australia
Kayode Ojo
University of Washington
Evripidis Gavathiotis
Professor
Albert Einstein College of Medicine
Van Hung Vuong Le
Københavns Universitet
Juan José Lasarte
Universidad de Navarra
Willem Joost Lesterhuis
University of Western Australia
Alexander Agoulnik
Florida International University
Angelo Taglialatela
Columbia University Irving Medical Center
Gareth Nealon
University of Western Australia
William Kerr
Professor of Microbiology & Immunology
SUNY Upstate Medical University
Isin Cakir
Assistant Professor of Medicine
University of Pittsburgh
Agnidipta Ghosh
Albert Einstein College of Medicine
Amber Boyatzis
University of Western Australia
Ariela Samantha
University of Western Australia
Mariana Lizeth Orozco Morales
University of Western Australia
Christian Weber
Chair in Vascular Medicine and Physician-in-Chief
LMU Klinikum
William Bradshaw
University of Oxford
Deirdre Coombe
Professor
Curtin University
Van Chi Thai
University of Western Australia
Matthew Piggott
University of Western Australia
Gemma Turner
University of Western Australia
Andrea Ilari
Istituto di Biologia e Patologia Molecolari Consiglio Nazionale delle Ricerche
David Siderovski
Professor and Chairman
University of North Texas Health Science Center
Hideo Nakano
Nagoya University
Jonathan So
Assistant Professor
Laura and Isaac Perlmutter Cancer Center at NYU Langone Health
Kirsikka Musta
Tampere University
Charlene M. Kahler
University of Western Australia
Silvia Buroni
Associate Professor
Università degli Studi di Pavia
Ashley L St. John
-
Megan O'Mara
Professor
University of Queensland
Rajkumar Verma
Assistant Professor
University of Connecticut School of Medicine
Monika Murcha
University of Western Australia
Robert Bradley
Full Professor
Fred Hutchinson Cancer Research Center
Mitali Sarkar‐Tyson
-
Yogesh Gupta
Associate Professor, with Tenure
University of Texas Health Science Center at San Antonio
Thomas Kampourakis
Lecturer
King’s College London
John Bruning
Senior Lecturer
University of Adelaide
William Brent Clayton
Associate Research Professor of Medicine
Indiana University School of Medicine
Jarrod Mousa
Assistant Professor
University of Georgia
Martin St. Maurice
Associate Professor
Marquette University
ApplyKite TwinLearns as you applyMake your next move.
Prepare your application
Review drafts before you send them.